灭活益生菌刺激印度鲮（Catla catla，汉密尔顿氏）的细胞免疫反应（体外实验）
Inactivated Probiotic Bacteria Stimulate Cellular Immune Responses of Catla, Catla catla (Hamilton) In Vitro.
作者信息
Kamilya Dibyendu, Baruah Arunjyoti, Sangma Timothy, Chowdhury Supratim, Pal Prasenjit
机构信息
Department of Fish Health and Environment, College of Fisheries, Central Agricultural University, Lembucherra, 799 210, Tripura, India,
出版信息
Probiotics Antimicrob Proteins. 2015 Jun;7(2):101-6. doi: 10.1007/s12602-015-9191-9.
In the present study, we investigated the in vitro immunostimulatory effects of inactivated form of two potential probiotics, Bacillus amyloliquefaciens FPTB16 and B. subtilis FPTB13 in catla (Catla catla). Catla head kidney leukocytes (HK) were incubated with the bacteria alive or inactivated with heat shock (2 h at 60 °C), UV light (for 2.5 h) and formalin (1.0%, v/v) treatment (for 24 h at 4 °C) at different concentrations (10(7), 10(8) and 10(9) cells ml(-1)). After incubation, different cellular immune parameters such as respiratory burst activity, nitric oxide production, leukocyte peroxidase content and proliferative response were analyzed. The inactivated probiotic preparations stimulated all the cellular immune parameters of catla HK leukocytes in vitro. Among the different inactivated preparations, heat-treated form exhibited the best result. The lowest dose (10(7) cells ml(-1)) of both the strains showed the maximum stimulation. The results collectively suggest the efficacy of inactivated preparations to be used as immunostimulant in aquaculture.
在本研究中，我们调查了两种潜在益生菌解淀粉芽孢杆菌FPTB16和枯草芽孢杆菌FPTB13的灭活形式对印度鲮（Catla catla）的体外免疫刺激作用。将印度鲮头肾白细胞（HK）与不同浓度（10⁷、10⁸和10⁹个细胞/毫升）的活细菌或经热休克（60℃处理2小时）、紫外线（处理2.5小时）和福尔马林（1.0%，v/v）处理（4℃处理24小时）灭活的细菌一起孵育。孵育后，分析了不同的细胞免疫参数，如呼吸爆发活性、一氧化氮产生、白细胞过氧化物酶含量和增殖反应。灭活的益生菌制剂在体外刺激了印度鲮HK白细胞的所有细胞免疫参数。在不同的灭活制剂中，热处理形式表现出最佳效果。两种菌株的最低剂量（10⁷个细胞/毫升）显示出最大刺激作用。这些结果共同表明灭活制剂作为水产养殖免疫刺激剂的有效性。
Zotero 插件