Suzuki Nobuo, Ogiso Shouzo, Yachiguchi Koji, Kawabe Kimi, Makino Fumiya, Toriba Akira, Kiyomoto Masato, Sekiguchi Toshio, Tabuchi Yoshiaki, Kondo Takashi, Kitamura Kei-ichiro, Hong Chun-Sang, Srivastav Ajai K, Oshima Yuji, Hattori Atsuhiko, Hayakawa Kazuichi
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Ishikawa 927-0553, Japan.
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Ishikawa 927-0553, Japan.
Comp Biochem Physiol C Toxicol Pharmacol. 2015 May;171:55-60. doi: 10.1016/j.cbpc.2015.02.004. Epub 2015 Feb 28.
We previously demonstrated that monohydroxylated polycyclic aromatic hydrocarbons (OHPAHs), which are metabolites of polycyclic aromatic hydrocarbons (PAHs), act on calcified tissue and suppress osteoblastic and osteoclastic activity in the scales of teleost fish. The compounds may possibly influence other calcified tissues. Thus, the present study noted the calcified spicules in sea urchins and examined the effect of both PAHs and OHPAHs on spicule formation during the embryogenesis of sea urchins. After fertilization, benz[a]anthracene (BaA) and 4-hydroxybenz[a]anthracene (4-OHBaA) were added to seawater at concentrations of 10(-8) and 10(-7) M and kept at 18 °C. The influence of the compound was given at the time of the pluteus larva. At this stage, the length of the spicule was significantly suppressed by 4-OHBaA (10(-8) and 10(-7) M). BaA (10(-7) M) decreased the length of the spicule significantly, while the length did not change with BaA (10(-8) M). The expression of mRNAs (spicule matrix protein and transcription factors) in the 4-OHBaA (10(-7) M)-treated embryos was more strongly inhibited than were those in the BaA (10(-7) M)-treated embryos. This is the first study to demonstrate that OHPAHs suppress spicule formation in sea urchins.
我们之前证明，多环芳烃（PAHs）的代谢产物单羟基化多环芳烃（OHPAHs）作用于钙化组织，并抑制硬骨鱼鳞片中的成骨细胞和破骨细胞活性。这些化合物可能会影响其他钙化组织。因此，本研究关注了海胆中的钙化针状体，并研究了PAHs和OHPAHs对海胆胚胎发育过程中针状体形成的影响。受精后，将苯并[a]蒽（BaA）和4-羟基苯并[a]蒽（4-OHBaA）以10^(-8)和10^(-7) M的浓度添加到海水中，并保持在18°C。在长腕幼虫阶段施加化合物的影响。在此阶段，4-OHBaA（10^(-8)和10^(-7) M）显著抑制了针状体的长度。BaA（10^(-7) M）显著降低了针状体的长度，而BaA（10^(-8) M）处理时长度没有变化。在4-OHBaA（10^(-7) M）处理的胚胎中，mRNA（针状体基质蛋白和转录因子）的表达比BaA（10^(-7) M）处理的胚胎受到更强的抑制。这是第一项证明OHPAHs抑制海胆针状体形成的研究。
