Wang Q-M, Theelen B, Groenewald M, Bai F-Y, Boekhout T
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;
CBS-KNAW Fungal Biodiversity Centre, Yeast Division, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Persoonia. 2014 Dec;33:41-7. doi: 10.3767/003158514X682313. Epub 2014 May 23.
Ustilaginomycotina (Basidiomycota, Fungi) has been reclassified recently based on multiple gene sequence analyses. However, the phylogenetic placement of two yeast-like genera Malassezia and Moniliella in the subphylum remains unclear. Phylogenetic analyses using different algorithms based on the sequences of six genes, including the small subunit (18S) ribosomal DNA (rDNA), the large subunit (26S) rDNA D1/D2 domains, the internal transcribed spacer regions (ITS 1 and 2) including 5.8S rDNA, the two subunits of RNA polymerase II (RPB1 and RPB2) and the translation elongation factor 1-α (EF1-α), were performed to address their phylogenetic positions. Our analyses indicated that Malassezia and Moniliella represented two deeply rooted lineages within Ustilaginomycotina and have a sister relationship to both Ustilaginomycetes and Exobasidiomycetes. Those clades are described here as new classes, namely Moniliellomycetes with order Moniliellales, family Moniliellaceae, and genus Moniliella; and Malasseziomycetes with order Malasseziales, family Malasseziaceae, and genus Malassezia. Phenotypic differences support this classification suggesting widely different life styles among the mainly plant pathogenic Ustilaginomycotina.
黑粉菌亚门（担子菌门，真菌）最近基于多个基因序列分析进行了重新分类。然而，两个酵母样属马拉色菌属和念珠霉属在该亚门中的系统发育位置仍不清楚。基于六个基因的序列，使用不同算法进行了系统发育分析，这些基因包括小亚基（18S）核糖体DNA（rDNA）、大亚基（26S）rDNA D1/D2结构域、包括5.8S rDNA的内部转录间隔区（ITS 1和2）、RNA聚合酶II的两个亚基（RPB1和RPB2）以及翻译延伸因子1-α（EF1-α），以确定它们的系统发育位置。我们的分析表明，马拉色菌属和念珠霉属代表黑粉菌亚门内两个根基深厚的谱系，并且与黑粉菌纲和外担子菌纲具有姐妹关系。这些分支在这里被描述为新的纲，即念珠霉纲，包含念珠霉目、念珠霉科和念珠霉属；以及马拉色菌纲，包含马拉色菌目、马拉色菌科和马拉色菌属。表型差异支持这一分类，表明在主要为植物病原的黑粉菌亚门中存在广泛不同的生活方式。
