Madhyastha Prashanthi Sampath, Naik Dilip G, Kotian Ravindra, Padma Divya, Srikant N, Bhat Kumar M R
Senior Grade Lecturer, Department of Dental Materials, Manipal College of Dental Sciences , Manipal University, Mangalore, India .
Professor, Department of Periodontics, Manipal College of Dental Sciences , Manipal University, Mangalore, India .
J Clin Diagn Res. 2015 Jan;9(1):ZC05-8. doi: 10.7860/JCDR/2015/10269.5366. Epub 2015 Jan 1.
The effects of leached substances from the restorative dental materials may induce local and systemic adverse effects. Thus the biological and toxic properties of the restorative dental materials must be compatible with the oral tissues or with general health. Therefore, the need for biocompatible restorative dental material implies the necessity of toxicity testing. It was the purpose of this investigation to determine and compare the possible toxic effect of silorane based composite (Filtek P90) on human gingival fibroblast (HGF) in vitro using cytotoxicity measuring parameters (MTT assay) in comparison with its methacrylate counterpart (Z100) for their viability, proliferation rate.
Fresh healthy biopsy specimens of human gingival tissue of patients were obtained. For HGF, cells were cultured in Dulbecco's modified Eagle medium and grown to sub confluent monolayers. After attaining confluence, cells were treated with different doses of the Filtek P90 or Z 100 for different time point. HGF cells were observed for their proliferation, viability by MTT assay.
The results of the cytotoxicity assay showed that, the percentage of viable cells was very good in the first 24h and marginally decreased in the next 48h period in all groups. However, the proliferation rate was never below 84% in all the groups, at any given concentration. Filtek P90 and Z100 treated cells exhibited insignificant decrease in the cell proliferation both in 24h and 48h exposure when compared to significant decrease in the cell survival rate in the positive control (Mitomycin C 250 μg/ml).) Comparison of the toxicity between Filtek P90 and Z100 in 24h & 48h separately showed that there was no significant difference (p<0.05) between these two composites in 24h and 48h' time period at all concentrations of the composites.
To conclude, the new silorane based restorative composite showed comparable cytotoxic characteristics to clinically successful dimethacrylate composites suggesting the non-toxic nature in the oral environment and hence contributing to clinical success of these new restorative materials.
牙科修复材料中浸出物质的影响可能会引发局部和全身不良反应。因此，牙科修复材料的生物学和毒性特性必须与口腔组织或整体健康状况相兼容。所以，对生物相容性牙科修复材料的需求意味着进行毒性测试的必要性。本研究的目的是使用细胞毒性测量参数（MTT 法），在体外测定并比较基于硅氧烷的复合材料（Filtek P90）与人牙龈成纤维细胞（HGF）的可能毒性作用，并与其甲基丙烯酸酯类对应材料（Z100）的活力、增殖率进行比较。
获取患者新鲜健康的人牙龈组织活检标本。对于 HGF，细胞在 Dulbecco 改良 Eagle 培养基中培养，生长至亚汇合单层。达到汇合后，在不同时间点用不同剂量的 Filtek P90 或 Z100 处理细胞。通过 MTT 法观察 HGF 细胞的增殖和活力。
细胞毒性试验结果表明，所有组在前 24 小时活细胞百分比非常高，在接下来的 48 小时略有下降。然而，在任何给定浓度下，所有组的增殖率从未低于 84%。与阳性对照（丝裂霉素 C 250μg/ml）中细胞存活率的显著下降相比，Filtek P90 和 Z100 处理的细胞在 24 小时和 48 小时暴露时细胞增殖的下降均不显著。分别比较 Filtek P90 和 Z100 在 24 小时和 48 小时的毒性表明，在复合材料的所有浓度下，这两种复合材料在 24 小时和 48 小时时间段内均无显著差异（p<0.05）。
总之，新型基于硅氧烷的修复性复合材料显示出与临床上成功的二甲基丙烯酸酯复合材料相当的细胞毒性特征，表明其在口腔环境中无毒，因此有助于这些新型修复材料的临床成功。
