Barakat Mohamed N, Saleh Mohamed, Al-Doss Abdullah A, Moustafa Khaled A, Elshafei Adel A, Al-Qurainy Fahed H
King Saud University Plant Production Department, College of Food and Agriculture Sciences Riyadh Saudi Arabia University of Alexandria Biotechnology Laboratory, Crop Science Department, Faculty of Agriculture Alexandria Egypt.
King Saud University Botany and Microbiology Department, College of Pure Science Riyadh Saudi Arabia.
Acta Biol Hung. 2015 Mar;66(1):93-102. doi: 10.1556/ABiol.66.2015.1.8.
Segregating F4 families from the cross between drought sensitive (Yecora Rojo) and drought tolerant (Pavon 76) genotypes were made to identify SSR markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat (Triticum aestivum L.) under water-stressed condition and to map quantitative trait locus (QTL) for the three physiological traits. The parents and 150 F4 families were evaluated phenotypically for drought tolerance using two irrigation treatments (2500 and 7500 m3/ha). Using 400 SSR primers tested for polymorphism in testing parental and F4 families genotypes, the results revealed that QTL for leaf chlorophyll content, flag leaf senescence and cell membrane stability traits were associated with 12, 5 and 12 SSR markers, respectively and explained phenotypic variation ranged from 6 to 42%. The SSR markers for physiological traits had genetic distances ranged from 12.5 to 25.5 cM. These SSR markers can be further used in breeding programs for drought tolerance in wheat.
从干旱敏感型（Yecora Rojo）和耐旱型（Pavon 76）基因型杂交后代中分离出F4家系，以鉴定与水分胁迫条件下小麦（Triticum aestivum L.）叶片叶绿素含量、旗叶衰老和细胞膜稳定性性状相关的SSR标记，并对这三个生理性状的数量性状位点（QTL）进行定位。利用两种灌溉处理（2500和7500立方米/公顷）对亲本和150个F4家系进行耐旱性表型评价。通过对400对SSR引物进行多态性检测，分析亲本和F4家系的基因型，结果表明，叶片叶绿素含量、旗叶衰老和细胞膜稳定性性状的QTL分别与12个、5个和12个SSR标记相关，解释的表型变异范围为6%至42%。生理性状的SSR标记遗传距离在12.5至25.5厘摩之间。这些SSR标记可进一步用于小麦耐旱性育种计划。
