Ren Yan, Sun Xiaonan, Nie Jingyun, Guo Peng, Wu Xiaohui, Zhang Yixiao, Gao Mengjuan, Niaz Mohsin, Yang Xia, Sun Congwei, Zhang Ning, Chen Feng
National Key Laboratory of Wheat and Maize Crop Science/Agronomy College/CIMMYT-China Wheat and Maize Joint Research Center, Henan Agricultural University, Zhengzhou, 450046 China.
Mol Breed. 2023 Aug 8;43(8):66. doi: 10.1007/s11032-023-01410-3. eCollection 2023 Aug.
Flag leaf senescence is a critical factor affecting the yield and quality of wheat. The aim of this study was to identify QTLs associated with flag leaf senescence in an F recombinant inbred line population derived from durum wheats UC1113 and Kofa. Bulked segregant analysis using the wheat 660K SNP array identified 3225 SNPs between extreme-phenotype bulks, and the differential SNPs were mainly clustered on chromosomes 1A, 1B, 3B, 5A, 5B, and 7A. BSR-Seq indicated that the significant SNPs were mainly located in two intervals of 354.0-389.0 Mb and 8.0-15.0 Mb on 1B and 3B, respectively. Based on the distribution of significant SNPs on chromosomes 1B and 3B, a total of 109 insertion/deletion (InDel) markers were developed, and 8 of them were finally used to map QTL in UC1113/Kofa population for flag leaf senescence. Inclusive composite interval mapping identified two major QTL in marker intervals Mar2005-Mar2116 and Mar207-Mar289, explaining 14.2-15.4% and 31.4-68.6% of the phenotypic variances across environments, respectively. Using BSR-Seq, gene expression and sequence analysis, the TraesCS1B02G211600 and TraesCS3B02G023000 were identified as candidate senescence-associated genes. This study has potential to be used in cloning key genes for flag leaf senescence and provides available molecular markers for genotyping and marker-assisted selection breeding.
The online version contains supplementary material available at 10.1007/s11032-023-01410-3.
旗叶衰老为影响小麦产量和品质的关键因素。本研究旨在鉴定源自硬粒小麦UC1113和Kofa的F重组自交系群体中与旗叶衰老相关的QTL。利用小麦660K SNP芯片进行混合分组分析法，在极端表型混合群体间鉴定出3225个SNP，差异SNP主要聚集在1A、1B、3B、5A、5B和7A染色体上。BSR-Seq分析表明，显著SNP分别主要位于1B染色体上354.0 - 389.0 Mb和3B染色体上8.0 - 15.0 Mb的两个区间。基于1B和3B染色体上显著SNP的分布，共开发了109个插入/缺失（InDel）标记，最终从中选取8个用于在UC1113/Kofa群体中对旗叶衰老进行QTL定位。采用完备复合区间作图法在标记区间Mar2005 - Mar2116和Mar207 - Mar289中鉴定出两个主要QTL，分别解释了不同环境下表型变异的14.2% - 15.4%和31.4% - 68.6%。通过BSR-Seq、基因表达和序列分析，将TraesCS1B02G211600和TraesCS3B02G023000鉴定为衰老相关候选基因。本研究有潜力用于克隆旗叶衰老关键基因，并为基因分型和标记辅助选择育种提供可用分子标记。
在线版本包含可在10.1007/s11032-023-01410-3获取的补充材料。
