Arenas Claudia Marcela, Gómez-Molina Andrea, Delgado Jean Paul
Grupo Genética, Regeneración y Cáncer, Instituto de Biología, Universidad de Antioquia, Calle 67 NO. 53-108, Medellín, Colombia.
Methods Mol Biol. 2015;1290:71-8. doi: 10.1007/978-1-4939-2495-0_5.
Limb regeneration studies have been extensively carried out in species of Ambystomatidae and Salamandridae families. So far limited research has been conducted in species belonging to the Plethodontidae family, where some of the species differs from other salamander families due to their direct development, thus absence of a larval life. Here, we describe a protocol to maintain the plethodontid salamanders of genus Bolitoglossa species under laboratory conditions to perform regeneration studies.
肢体再生研究已在钝口螈科和蝾螈科物种中广泛开展。到目前为止，对属于无肺螈科的物种开展的研究有限，该科的一些物种由于直接发育，即没有幼体阶段，因而与其他蝾螈科不同。在此，我们描述了一种在实验室条件下饲养无肺螈属物种以进行再生研究的方案。
