利用CRISPR-Cas9系统高效编辑痘苗病毒基因组。
Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system.
作者信息
Yuan Ming, Zhang Wensheng, Wang Jun, Al Yaghchi Chadwan, Ahmed Jahangir, Chard Louisa, Lemoine Nick R, Wang Yaohe
机构信息
Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
Cambridge Suda Genome Research Center, Soochow University, Soochow, Jiangsu Province, People's Republic of China Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
出版信息
J Virol. 2015 May;89(9):5176-9. doi: 10.1128/JVI.00339-15. Epub 2015 Mar 4.
Abstract
Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.
摘要
自牛痘病毒（VACV）被用于根除天花以来，它一直被用于免疫疗法以预防传染病和治疗癌症。然而，目前编辑VACV基因组的方法效率不高。在此，我们证明CRISPR-Cas9系统可用于快速有效地编辑VACV基因组。此外，一组针对所有VACV基因的8964个通过计算设计的独特导向RNA（gRNA）对于VACV基因功能的研究将具有重要价值。
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