Zingg H H, Goodman R H, Habener J F
Endocrinology. 1984 Jul;115(1):90-4. doi: 10.1210/endo-115-1-90.
The developmental expression of the somatostatin (SRIF) gene was investigated in rat brain and stomach, two SRIF-rich tissues. The accumulation of mRNA encoding SRIF was determined in these organs during fetal and early (1-4 weeks) postnatal development using a sensitive radiodensitometric cDNA hybridization assay and a cloned preprosomatostatin cDNA. A single band of mRNA which hybridized specifically to the rat SRIF cDNA was detected in both tissues examined throughout ontogenesis, suggesting that the same SRIF gene is expressed in these tissues in the developing as well as in the adult rat. Whereas SRIF mRNA was undetectable in fetal stomach and rose gradually only after birth, brain SRIF mRNA was already detectable by day 7 of embryonic life and reached concentrations corresponding to those in the adult brain by embryonic day 20. These marked differences may reflect basic differences in the developmental regulation of SRIF gene expression in neural vs. nonneural tissues or may be related to the onset of functional activity in the organs studied.
在大鼠脑和胃这两个富含生长抑素（SRIF）的组织中，对生长抑素（SRIF）基因的发育表达进行了研究。在胎儿期和出生后早期（1 - 4周）发育阶段，使用灵敏的放射密度测定cDNA杂交分析法和克隆的前促生长抑素cDNA，测定这些器官中编码SRIF的mRNA的积累情况。在整个个体发育过程中检查的两种组织中均检测到一条与大鼠SRIF cDNA特异性杂交的mRNA条带，这表明在发育中的大鼠以及成年大鼠的这些组织中表达的是相同的SRIF基因。胎儿胃中未检测到SRIF mRNA，出生后才逐渐升高，而脑SRIF mRNA在胚胎期第7天就已可检测到，并在胚胎期第20天达到与成年脑相对应的浓度。这些显著差异可能反映了神经组织与非神经组织中SRIF基因表达发育调控的基本差异，或者可能与所研究器官中功能活动的开始有关。
