Norén O, Dabelsteen E, Høyer P E, Olsen J, Sjöström H, Hansen G H
Department of Biochemistry C, University of Copenhagen, Denmark.
FEBS Lett. 1989 Dec 18;259(1):107-12. doi: 10.1016/0014-5793(89)81506-6.
The sequence of a cDNA clone (2.82 kbp) of rabbit intestinal aminopeptidase N (CD 13) is reported. Using the corresponding anti-sense RNA probe, the distribution of aminopeptidase N mRNA along the crypt/villus axis of the rabbit small intestine was studied by in situ hybridization. The aminopeptidase N gene is expressed along the whole length of the villus with a maximum at its base. Expression was not detected in the crypt cells. The distribution of aminopeptidase N mRNA correlates with the presence of active enzyme as monitored by histochemical staining. The results are compatible with onset of transcription of the aminopeptidase N gene at the crypt/villus transition zone during the enterocyte differentiation.
报道了兔肠道氨肽酶N（CD 13）的一个cDNA克隆（2.82kbp）的序列。使用相应的反义RNA探针，通过原位杂交研究了氨肽酶N mRNA在兔小肠隐窝/绒毛轴上的分布。氨肽酶N基因在绒毛全长均有表达，在其基部表达量最高。在隐窝细胞中未检测到表达。通过组织化学染色监测，氨肽酶N mRNA的分布与活性酶的存在相关。这些结果与在肠上皮细胞分化过程中氨肽酶N基因在隐窝/绒毛过渡区开始转录相一致。
