Olsen J, Sjöström H, Norén O
Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark.
FEBS Lett. 1989 Jul 17;251(1-2):275-81. doi: 10.1016/0014-5793(89)81470-x.
We have isolated four lambda-phages covering the complete pig aminopeptidase N/CD13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5' upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons we have found three domains showing similarity to promoter regions of the genes encoding human alpha 1-antitrypsin and human intestinal alkaline phosphatase. The gene sequence includes the first three exons and two introns. It shows that a single exon encodes the cytoplasmic tail, the membrane anchor and the junctional peptide.
我们分离出了四个覆盖完整猪氨肽酶N/CD13基因的λ噬菌体。2.85kbp的序列包含1.18kbp的5'上游区域和1.67kbp的结构基因。在启动子区域，我们发现了一个TATA盒以及CTF-1/NF-1和AP-2的潜在结合位点。通过序列比较，我们发现了三个与编码人α1-抗胰蛋白酶和人肠碱性磷酸酶的基因启动子区域相似的结构域。该基因序列包括前三个外显子和两个内含子。结果表明，单个外显子编码细胞质尾巴、膜锚定结构域和连接肽。
