Vongkulsiri Sritatath, Suzuki Mihoko, Spaide Richard F
*Vitreous Retina Macula Consultants of New York, New York, New York; and †LuEsther T. Mertz Retinal Research Center, Manhattan Eye, Ear and Throat Hospital, New York, New York.
Retina. 2015 Jun;35(6):1211-5. doi: 10.1097/IAE.0000000000000514.
To examine the colocalization error between the infrared reflectance (IR) scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SD-OCT) images of the Heidelberg Spectralis.
The IR and corresponding horizontal raster SD-OCT images were compared in 10 healthy volunteers examined with 3 Heidelberg Spectralis + OCT instruments. The center points of retinal vessels selected by random uniform sampling in scanning laser ophthalmoscope-IR images were compared with colocalizing points in corresponding SD-OCT images by two masked readers. The error of colocalization was measured in the SD-OCT image. The point positions were recorded using Cartesian coordinates measured in microns. The error of colocalization was evaluated using a fixed-effects generalized least squares regression model with location and instrument as predictor variables.
A total of 1,617 points in the IR and SD-OCT images were analyzed, and the mean error of colocalization was 42.2 ± 32.4 μm. The Heidelberg Spectralis instrument used was not a significant predictor of colocalization error (P = 0.15). The colocalization errors were negatively correlated with x-coordinate position (P < 0.001).
There is a mean colocalization error between the IR and SD-OCT images produced by the Heidelberg Spectralis that is on the scale of many features being evaluated in the fundus. The variability in these measurements means the confidence interval for the exact colocalization is much larger. Because of the magnitude and variability of the error, the colocalization feature of the Heidelberg Spectralis should be used as a rough guide, not an absolute determinant.
检测海德堡Spectralis的红外反射（IR）扫描激光检眼镜（SLO）与光谱域光学相干断层扫描（SD-OCT）图像之间的共定位误差。
对10名健康志愿者使用3台海德尔堡Spectralis + OCT仪器进行检查，比较IR图像和相应的水平光栅SD-OCT图像。两名经过掩蔽的阅片者将扫描激光检眼镜-IR图像中通过随机均匀采样选择的视网膜血管中心点与相应SD-OCT图像中的共定位点进行比较。在SD-OCT图像中测量共定位误差。使用以微米为单位测量的笛卡尔坐标记录点的位置。使用以位置和仪器作为预测变量的固定效应广义最小二乘回归模型评估共定位误差。
共分析了IR图像和SD-OCT图像中的1617个点，共定位的平均误差为42.2±32.4μm。所使用的海德堡Spectralis仪器不是共定位误差的显著预测因素（P = 0.15）。共定位误差与x坐标位置呈负相关（P < 0.001）。
海德堡Spectralis产生的IR图像和SD-OCT图像之间存在平均共定位误差，该误差处于眼底许多被评估特征的尺度范围内。这些测量中的变异性意味着精确共定位的置信区间要大得多。由于误差的大小和变异性，海德堡Spectralis的共定位特征应作为一个粗略的指导，而不是绝对的决定因素。
