El-Sharkawy Eman R, Mahmoud Khaled
a Department of Eco-physiology , Desert Research Center , 15753 Cairo , Egypt.
b Chemical Department , Faculty of Science, Northern Borders University , Arar , Kingdom of Saudi Arabia.
Nat Prod Res. 2016;30(4):394-8. doi: 10.1080/14786419.2015.1017492. Epub 2015 Mar 9.
The chloroform fraction of methanol (MeOH) extract of the aerial parts of Launaea mucronata was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50 = 20.0, 18.6 and 14.30 μg/mL, respectively. The chloroform fraction of the MeOH extract was subjected to further fractionation, which led to the isolation of two new coumarin compounds (6-isobutyl coumarin and 6-isobutyl-7-methyl- coumarin). The structures of the new compounds were elucidated by high field 1D and 2D NMR and ESI-MS spectroscopies. Low polar fractions revealed the isolation of other known triterpene compounds which were identified according to its spectral data and comparison with the literature. New coumarin compounds show high cytotoxicity against MCF-7, HCT116 and HepG2 cell lines.
对多裂山莴苣地上部分甲醇提取物的氯仿馏分进行了体外细胞毒性研究，考察其对HCT116、HepG2和MCF-7细胞系的作用，结果其IC50分别为20.0、18.6和14.30 μg/mL。对甲醇提取物的氯仿馏分进行进一步分离，得到两种新的香豆素化合物（6-异丁基香豆素和6-异丁基-7-甲基香豆素）。通过高场一维和二维核磁共振及电喷雾电离质谱对新化合物的结构进行了鉴定。低极性馏分中分离出了其他已知的三萜类化合物，根据其光谱数据并与文献比较进行了鉴定。新的香豆素化合物对MCF-7、HCT116和HepG2细胞系显示出高细胞毒性。
