Elsharkawy Eman R
Department of Environment and plant pasture, Desert Research Center, Mathef El-Mataria, Matariya, Cairo, Egypt / Department of Chemistry, Science Faculty, Girls department, Northern Border University-ARAR, North Region, Saudi Arabia.
Pak J Pharm Sci. 2017 Mar;30(2):399-405.
Traditional Medicine is widely used in clinical research due to its low toxicity, low number of side effects, and low cost. Many components of common fruits and vegetables play well-documented roles as chemo preventive or chemotherapeutic agents that suppress tumor genesis. The present study was aimed to identify the DPPH (2, 2-diphenyl-1-picrylhydrazyl) free-radical scavenging constituents from methanol extract of Launaea mucronata using bioassay-guided fractionation. The methanolic extract revealed a strong antioxidant activity, the IC50 values (the concentration with scavenging activity of 50%) was 25.94 mg/ml. The fractionation of the active methanol extract by silica gel column chromatography revealed compounds with strong antioxidant activity. The isolated bioactive compound was determined as, apigenin, quercetin, rutin, isorhamnetin-3-O-β-glucoside, quercetin-3-O-β-glucoside, apigenin-7-O- β-diglucoside, kaempferol-3-o-rutinoside), by comparing spectral data (UV, IR, H NMR, C NMR, and MS) with literature reports. The results obtained confirmed that methanol extract of Launaea mucronata or its derived phytocompounds can be used potentially as a bioactive source of natural antioxidants by contributing beneficial health effects. Launaea mucronata was in vitro investigated for cytotoxic activity against HCT116, HepG2, Hella and MCF-7 cell lines, doxorubicin was used as the reference drug. The results show IC 19, 19.60, 12.60 and 12.70 μg/ml respectively.
传统医学因其低毒性、低副作用和低成本而广泛应用于临床研究。常见水果和蔬菜中的许多成分作为化学预防或化疗药物发挥着有充分文献记载的作用，可抑制肿瘤发生。本研究旨在通过生物测定导向分离法，从尖叶栓果菊甲醇提取物中鉴定出清除2,2-二苯基-1-苦基肼（DPPH）自由基的成分。甲醇提取物显示出较强的抗氧化活性，其IC50值（清除活性达50%时的浓度）为25.94 mg/ml。通过硅胶柱色谱法对活性甲醇提取物进行分离，得到了具有较强抗氧化活性的化合物。通过将光谱数据（紫外、红外、氢核磁共振、碳核磁共振和质谱）与文献报道进行比较，确定分离出的生物活性化合物为芹菜素、槲皮素、芦丁、异鼠李素-3-O-β-葡萄糖苷、槲皮素-3-O-β-葡萄糖苷、芹菜素-7-O-β-二葡萄糖苷、山柰酚-3-O-芸香糖苷。所得结果证实，尖叶栓果菊甲醇提取物或其衍生的植物化合物通过产生有益的健康影响，有可能作为天然抗氧化剂的生物活性来源。对尖叶栓果菊进行了体外研究，考察其对HCT116、HepG2、Hela和MCF-7细胞系的细胞毒性活性，以阿霉素作为参比药物。结果显示IC值分别为19、19.60、12.60和12.70 μg/ml。
