Moustafa E S, Swilam N F, Ghanem O B, Hashim A N, Nawwar M A, Lindequist U, Linscheid M W
Pharmazie. 2018 Apr 2;73(4):241-243. doi: 10.1691/ph.2018.7946.
Phenolic metabolite profiling using two dimensional paper chromatographic analysis (2 DPC) was used for assaying the complex mixture of phenolics of an aqueous ethanol aerial part extract of Cuphea ignea (Lytheraceae). A coumarin with a rare structure, namely, 7-hydroxy 3-methoxy coumarin 5-O-β-glucopyranoside was isolated from the investigated extract. The structure was elucidated by conventional methods and spectral analysis, including one and two dimensional NMR (1D and 2D NMR), as well as by interpretation of the spectra obtained by high resolution electrospray ionization mass technique (HRESIMS). The rare coumarin significantly inhibited reactive oxygen species production with an ED50 value of 6.31±1.64 μg/ml and 5.78±0.66 μg/ml as determined by the the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the oxygen radical absorption capacity (ORAC) assay respectively. The isolated coumarin presented a cytotoxic activity assessed by using the neutral red assay (NRU) against lung cancer cell line (H23) with IC50 of 40.38±2.75 μg/ml.
采用二维纸色谱分析（2DPC）对萼距花（千屈菜科）乙醇水提取物中的酚类化合物复杂混合物进行分析。从该提取物中分离出一种结构罕见的香豆素，即7-羟基-3-甲氧基香豆素-5-O-β-D-吡喃葡萄糖苷。通过常规方法和光谱分析，包括一维和二维核磁共振（1D和2D NMR）以及高分辨率电喷雾电离质谱技术（HRESIMS）获得的光谱解释来阐明其结构。这种罕见的香豆素通过自由基2,2-二苯基-1-苦基肼（DPPH）和氧自由基吸收能力（ORAC）测定法分别以6.31±1.64μg/ml和5.78±0.66μg/ml的半数有效剂量（ED50）显著抑制活性氧的产生。通过中性红试验（NRU）评估，分离出的香豆素对肺癌细胞系（H23）具有细胞毒性活性，半数抑制浓度（IC50）为40.38±2.75μg/ml。
