Fang Junwei, Zhang Guohua, Teng Xianmu, Zhang Zhiqin, Pan Jinhui, Shou Qiyang, Chen Minli
Xianju County Hospital of TCM, Taizhou 310053, China.
Zhonghua Gan Zang Bing Za Zhi. 2015 Jan;23(1):59-63. doi: 10.3760/cma.j.issn.1007-3418.2015.01.014.
To observe the effects ofGynura segetum in rats with hepatic veno-occlusive disease (HVOD).
Sixty Sprague-Dawley rats were assigned to a blank control group, one of three Gynura segetum treatment groups (low-dose group, 5.0 g/kg; mid-dose group, 10 g/kg; high-dose group, 20 g/kg), or a pseudo-drug group (10 g/kg of pseudo-ginseng). After 28 days of treatment, effects on white blood cell count, coagulation, secreted factors from vascular endothelium, and histopathology of the spleen were observed and inter-group differences were statistically assessed.
After the 4-week administration, all rats in the Gynura segetum treatment groups showed decrease in body weight, increases in numbers of leukocytes, neutrophils, lymphocytes, monocytes and eosinophils.decreases in platelets and platelet hematocrit, and increases in mean platelet volume and platelet distribution.In addition, the Gynura segetum treatments increased the prothrombin time, activated partial thromboplastin time, thrombin time, prothrombin ratio and international normalized ratio, but decreased the PT%, fibrinogen level and platelet aggregation.Serum levels of endothelin and nitric oxide were also elevated by the Gynura segetum treatments.All measured parameters showed significant differences from the control group (P less than 0.01 or less than 0.05).Finally, the splenic follicles were significantly reduced and the spleens showed an absence of germinal centers along with a large number of diffuse lymphocytes and reduced red pulp sinusoids.
The Gynura segetum treatment has some toxic effects; it can reduce platelet count and platelet hematocrit, inhibit blood clotting time and platelet aggregation, increase the secretion of factors from the vascular endothelium and disrupt spleen histology.
观察白凤菜对肝静脉闭塞病（HVOD）大鼠的影响。
将60只Sprague-Dawley大鼠分为空白对照组、三个白凤菜治疗组之一（低剂量组，5.0 g/kg；中剂量组，10 g/kg；高剂量组，20 g/kg）或假药物组（10 g/kg人参）。治疗28天后，观察对白细胞计数、凝血、血管内皮分泌因子及脾脏组织病理学的影响，并对组间差异进行统计学评估。
给药4周后，白凤菜治疗组所有大鼠体重下降，白细胞、中性粒细胞、淋巴细胞、单核细胞和嗜酸性粒细胞数量增加，血小板和血小板压积降低，平均血小板体积和血小板分布增加。此外，白凤菜治疗使凝血酶原时间、活化部分凝血活酶时间、凝血酶时间、凝血酶原比值和国际标准化比值增加，但PT%、纤维蛋白原水平和血小板聚集降低。白凤菜治疗还使血清内皮素和一氧化氮水平升高。所有测量参数与对照组均有显著差异（P小于0.01或小于0.05）。最后，脾滤泡明显减少，脾脏生发中心缺失，伴有大量弥漫性淋巴细胞，红髓血窦减少。
白凤菜治疗有一定毒性作用；可降低血小板计数和血小板压积，抑制凝血时间和血小板聚集，增加血管内皮因子分泌并破坏脾脏组织学结构。
