Department of Digestive Disease, The Second Xiang-Ya Hospital of Central South University, Changsha, Hunan Province, China.
J Gastroenterol Hepatol. 2011 Jun;26(6):1016-21. doi: 10.1111/j.1440-1746.2011.06661.x.
To investigate the therapeutic effect of ligustrazine on hepatic veno-occlusive disease (HVOD) induced by Gynura segetum and the possible mechanism of it.
Female Kunming mice (115) were randomly divided into four groups, gavaged with 30 g/kg per day Gynura segetum (group A), 30 g/kg per day Gynura segetum + 100 mg/kg per day ligustrazine (group B), 30 g/kg per day Gynura segetum + 200 mg/kg per day ligustrazine (group C) or 30 mL/kg per day phosphate-buffered saline (PBS) (group D). Thirty days later, all of the mice were killed. Blood samples and livers were harvested. Histological changes were evaluated by light microscopy. Liver function was measured, and the expression of tissue factor (TF), early growth response factor-1 (Egr-1) and nuclear factor-KBp65 (NF-KBp65) were determined by reverse transcription-polymerase chain reaction and Western blot.
A total of 24 mice in group A developed HVOD. Compared with the controls, they had increased liver ratio, serum total bilirubin (TBIL), direct bilirubin (DBIL), transaminase and decreased albumin (ALB) (P < 0.05). Administration of ligustrazine improved the clinical signs and biochemistry parameters in a dose-dependent manner. Compared with group A, the expression of TF, Egr-1 and NF-KB p65 decreased in groups B and C (P < 0.05).
Ligustrazine has a therapeutic effect on HVOD, improving clinical manifestations and liver function. The possible mechanism may be that ligustrazine could reduce the expression of TF by downregulating the expression of transcription factors: Egr-1 and NF-KB p65.
研究川芎嗪对葛花致肝静脉闭塞病（HVOD）的治疗作用及其可能机制。
将 115 只昆明雌性小鼠随机分为 4 组，分别灌胃 30 g/kg 葛花（A 组）、30 g/kg 葛花+100 mg/kg 川芎嗪（B 组）、30 g/kg 葛花+200 mg/kg 川芎嗪（C 组）或 30 mL/kg 磷酸盐缓冲液（PBS）（D 组），30 天后处死小鼠，取血及肝脏，光镜下观察组织学变化，检测肝功能，反转录-聚合酶链反应和 Western blot 法检测组织因子（TF）、早期生长反应因子-1（Egr-1）和核因子-κB 抑制蛋白 p65（NF-KBp65）的表达。
A 组共 24 只小鼠发生 HVOD，肝重比、血清总胆红素（TBIL）、直接胆红素（DBIL）、转氨酶升高，白蛋白（ALB）降低（P<0.05）；川芎嗪呈剂量依赖性改善上述临床及生化指标；与 A 组比较，B、C 组 TF、Egr-1 和 NF-KB p65 表达降低（P<0.05）。
川芎嗪对 HVOD 有治疗作用，可改善临床症状和肝功能，其机制可能与下调转录因子 Egr-1、NF-KB p65 的表达，减少 TF 表达有关。
