Kaya Hidetaka, Iwano Megumi, Takeda Seiji, Kanaoka Masahiro M, Kimura Sachie, Abe Mitsutomo, Kuchitsu Kazuyuki
a Department of Applied Biological Science ; Tokyo University of Science ; Noda , Japan.
Plant Signal Behav. 2015;10(2):e989050. doi: 10.4161/15592324.2014.989050.
Reactive oxygen species (ROS) accumulate at the tip of growing pollen tubes. In Arabidopsis, NADPH oxidases RbohH and RbohJ are localized at the plasma membrane of pollen tube tip and produce ROS in a Ca(2+)-dependent manner. The ROS produced by Rbohs and Ca(2+) presumably play a critical role in the positive feedback regulation that maintains the tip growth. Ultrastructural cytochemical analysis revealed ROS accumulation in the apoplast/cell wall of the pollen grains on the stigmatic papillae in the wild type, but not in the rbohH rbohJ double mutant, suggesting that apoplastic ROS derived from RbohH and RbohJ are involved in pollen tube elongation into the stigmatic papillae by affecting the cell wall metabolism.
活性氧（ROS）在生长中的花粉管顶端积累。在拟南芥中，NADPH氧化酶RbohH和RbohJ定位于花粉管顶端的质膜上，并以Ca(2+)依赖的方式产生ROS。Rbohs产生的ROS和Ca(2+)可能在维持顶端生长的正反馈调节中起关键作用。超微结构细胞化学分析显示，野生型中活性氧在柱头乳突上花粉粒的质外体/细胞壁中积累，而在rbohH rbohJ双突变体中则没有，这表明源自RbohH和RbohJ的质外体ROS通过影响细胞壁代谢参与花粉管向柱头乳突的伸长。
