Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety (VESPA), Università degli Studi di Milano, 20133 Milan, Italy.
Department of Environmental Science and Policy, Università degli Studi di Milano, 20133 Milan, Italy.
Genes (Basel). 2021 Apr 3;12(4):525. doi: 10.3390/genes12040525.
This review explores the role of reactive oxygen species (ROS)/Ca in communication within reproductive structures in plants and animals. Many concepts have been described during the last years regarding how biosynthesis, generation products, antioxidant systems, and signal transduction involve ROS signaling, as well as its possible link with developmental processes and response to biotic and abiotic stresses. In this review, we first addressed classic key concepts in ROS and Ca signaling in plants, both at the subcellular, cellular, and organ level. In the plant science field, during the last decades, new techniques have facilitated the in vivo monitoring of ROS signaling cascades. We will describe these powerful techniques in plants and compare them to those existing in animals. Development of new analytical techniques will facilitate the understanding of ROS signaling and their signal transduction pathways in plants and mammals. Many among those signaling pathways already have been studied in animals; therefore, a specific effort should be made to integrate this knowledge into plant biology. We here discuss examples of how changes in the ROS and Ca signaling pathways can affect differentiation processes in plants, focusing specifically on reproductive processes where the ROS and Ca signaling pathways influence the gametophyte functioning, sexual reproduction, and embryo formation in plants and animals. The study field regarding the role of ROS and Ca in signal transduction is evolving continuously, which is why we reviewed the recent literature and propose here the potential targets affecting ROS in reproductive processes. We discuss the opportunities to integrate comparative developmental studies and experimental approaches into studies on the role of ROS/ Ca in both plant and animal developmental biology studies, to further elucidate these crucial signaling pathways.
这篇综述探讨了活性氧（ROS）/Ca 在动植物生殖结构内通讯中的作用。在过去的几年中，已经描述了许多关于生物合成、产物生成、抗氧化系统和信号转导如何涉及 ROS 信号以及其与发育过程和对生物和非生物胁迫的反应可能存在联系的概念。在这篇综述中，我们首先介绍了植物中 ROS 和 Ca 信号转导的经典关键概念，包括亚细胞、细胞和器官水平。在植物科学领域，在过去的几十年中，新技术促进了 ROS 信号级联的体内监测。我们将描述这些在植物中强大的技术，并将其与动物中现有的技术进行比较。新分析技术的发展将有助于理解植物和哺乳动物中的 ROS 信号及其信号转导途径。其中许多信号途径已经在动物中进行了研究；因此，应该特别努力将这些知识整合到植物生物学中。我们在这里讨论了 ROS 和 Ca 信号通路的变化如何影响植物分化过程的例子，特别关注生殖过程，其中 ROS 和 Ca 信号通路影响植物和动物的配子体功能、有性生殖和胚胎形成。ROS 和 Ca 在信号转导中的作用的研究领域在不断发展，因此我们综述了最近的文献，并提出了影响生殖过程中 ROS 的潜在靶点。我们讨论了将比较发育研究和实验方法整合到 ROS/Ca 在植物和动物发育生物学研究中的作用研究中的机会，以进一步阐明这些关键信号途径。
