Conner-Kerr Teresa, Malpass Gloria, Steele Arhalia, Howlett Allyn
University of North Georgia, Dahlonega, GA;
Wake Forest University Health Sciences, Winston-Salem, NC.
Ostomy Wound Manage. 2015 Mar;61(3):34-41.
Low-frequency ultrasound (LFU) in the frequency range 30-40 kHz administered using different delivery methods (waterbath and noncontact spray) has shown positive effects on chronic wound healing rates in humans, but the underlying mechanisms are only beginning to be explored. To examine the effects of LFU delivered at 35 kHz on the morphology and migration of human fibroblasts, real-time videography was used to record the rate and character of cultured human fibroblast migration at 8-hour increments for 48 hours in a wound assay model. Cells were treated with 35 kHz LFU or saline only (control). Cellular morphology (cell shape, packing, and apparent length) and migration patterns including orientation were analyzed using time-lapse videography. LFU delivered at a frequency of 35 kHz produced a different pattern of fibroblast migration in vitro (varied orientation versus vertical orientation for untreated cells) and altered cell morphology compared to controls. The observed pattern of migration was more varied and widely distributed across multiple angles versus the predominant parallel orientation of the migrating untreated cells. The potential implications of these findings on collagen placement in the extracellular matrix, which may affect degree of soft tissue scarring, should be further investigated.
使用不同传递方式（水浴和非接触喷雾）施加的频率范围在30 - 40千赫兹的低频超声（LFU）已显示出对人类慢性伤口愈合率有积极影响，但其潜在机制才刚刚开始被探索。为了研究以35千赫兹施加的LFU对人成纤维细胞形态和迁移的影响，在伤口检测模型中，使用实时摄像以8小时为增量记录培养的人成纤维细胞48小时的迁移速率和特征。细胞分别用35千赫兹的LFU或仅用生理盐水（对照）处理。使用延时摄像分析细胞形态（细胞形状、堆积和表观长度）以及包括方向在内的迁移模式。与对照相比，以35千赫兹频率施加的LFU在体外产生了不同的成纤维细胞迁移模式（未处理细胞为垂直方向，处理后方向多样）并改变了细胞形态。观察到的迁移模式比未处理的迁移细胞主要的平行方向更加多样且分布在多个角度。这些发现对细胞外基质中胶原蛋白沉积的潜在影响，可能会影响软组织瘢痕形成的程度，应进一步研究。
