Zhao Yun-Sheng, Liu Xiu, Mao Fu-Ying, Tian Hong-Ling, Wan De-Guang
Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(20):3991-4000.
To establish an HPLC fingerprint to evaluate the quality of Polygalae Radix, root xylem, and those collected in different growth ages or harvest time.
Separation was performed at 30 °C on a Kromasil C18 column (4.6 mm x 250 mm, 5 μm); the mobile phases was acetonitrile and 0.05% H3PO4 water in the gradient elution; the flow rate was set at 1.0 mL · min(-1) and the detection wavelength at 314 nm; the quality discriminant analyses were accomplished by means of similarity analysis, cluster analysis, principal component analysis and neural network model.
In 26 batches of Polygalae Radix, 24 batches fingerprint similarities were above 0.8. In 5 different growth or harvest time batches, 4 batches were above 0.8; in 8 batches root xylem samples, the similarities were all above 0.875. The similarity analysis was in accord with the quality discriminant analysis of cluster analysis, principal component analysis and neural network model.
Fingerprint combined with chemical pattern recognition technique can effectively evaluate the quality of Polygalae Radix. The active substance species are all similar in cultivated, wild, different growth or harvest time Polygalae Radix and polygala root xylem, but the chromatography peak areas are different. The effective material contents are similar between wild and cultivated Polygalae Radix, but each chromatographic peak area of the root xylem is much smaller than that of Polygalae Radix. The chemical substance accumulation mainly depends on harvest month, but little growth time in Polygalae Radix.
建立高效液相色谱指纹图谱，以评价远志药材、根木质部以及不同生长年限或采收期所采集样品的质量。
采用Kromasil C18柱（4.6 mm×250 mm，5 μm），柱温30℃；流动相为乙腈和0.05%磷酸水溶液，梯度洗脱；流速设定为1.0 mL·min⁻¹，检测波长为314 nm；通过相似度分析、聚类分析、主成分分析和神经网络模型进行质量判别分析。
26批远志药材中，24批指纹图谱相似度在0.8以上。5个不同生长或采收期的批次中，4批相似度在0.8以上；8批根木质部样品的相似度均在0.875以上。相似度分析结果与聚类分析、主成分分析和神经网络模型的质量判别分析结果一致。
指纹图谱结合化学模式识别技术可有效评价远志药材质量。栽培远志、野生远志、不同生长或采收期远志及远志根木质部中的活性物质种类基本相似，但色谱峰面积存在差异。野生远志与栽培远志的有效物质含量相近，但根木质部各色谱峰面积均远小于远志药材。化学物质的积累主要取决于采收月份，而远志生长时间对其影响较小。
