Yang Hong-Xia, Ma Fang, Du Yu-Zhi, Sun Su-Qin, Wei Li-Xin
Guang Pu Xue Yu Guang Pu Fen Xi. 2014 Nov;34(11):2973-7.
The objective of the present study is to research the herb of Swertia mussotii Franch and its different extracts by tristep infrared spectroscopy. The main constitute of Swertia mussotii Franch-gentiamarin, which is also the higher content constitute, was selected as the control components to analyze the infrared spectroscopy and second derivative infrared spectroscopy of different extracts of Swertia mussotii Franch, at the same time, the different concentration of ethanol extracts were also analyzed by two-dimensional correlation spectroscopy (2D-COS). The results indicated that the intensity of 1 611 and 1 075 cm(-1) of gentiamarin, which are its two main absorptions in the infrared spectra, has the positive correlation with the content change in different extracts. The infrared spectroscopy of extracts are similar if the polarity of extract solvents is close; with the decreases in solution polarity, the intensity of 2 853, 1 733, 1 464, 1 277 and 1 161 cm(-1) in infrared spectroscopy of different extracts is increased, the content of esters and the extraction percentage terpenoid compounds are also increased; the different concentration of ethanol extracts has obviously difference when they are analyzed by two-dimensional correlation spectroscopy (2D-COS). The positive correlation between the intensity of absorptions and the content of the gentiamarin indicates that the infrared spectroscopy can reflect the content change in constitute; the similar and the change trend of the different concentrations of ethanol extract infrared spectroscopy approve the scientificalness of decoction of traditional medicine; infrared spectroscopy that used in the research can be used as an accurate, rapid and effective method in the pharmacological activity tests of transitional herbal Swertia mussotii F. and it's different extracts, even in the research on the tibetan medicine.
本研究旨在通过三步红外光谱法对川西獐牙菜及其不同提取物进行研究。选取川西獐牙菜的主要成分龙胆苦苷（也是含量较高的成分）作为对照品，分析川西獐牙菜不同提取物的红外光谱和二阶导数红外光谱，同时对不同浓度的乙醇提取物进行二维相关光谱（2D - COS）分析。结果表明，龙胆苦苷在红外光谱中的两个主要吸收峰1611和1075 cm⁻¹的强度与不同提取物中的含量变化呈正相关。提取物溶剂极性相近时，提取物的红外光谱相似；随着溶液极性降低，不同提取物红外光谱中2853、1733、1464、1277和1161 cm⁻¹处的强度增加，酯类含量和萜类化合物提取率也增加；不同浓度的乙醇提取物经二维相关光谱（2D - COS）分析时有明显差异。吸收峰强度与龙胆苦苷含量之间的正相关表明红外光谱能够反映成分含量变化；不同浓度乙醇提取物红外光谱的相似性及变化趋势证实了传统药物煎煮的科学性；本研究中使用的红外光谱法可作为一种准确、快速且有效的方法用于传统草药川西獐牙菜及其不同提取物的药理活性测试，甚至在藏药研究中也适用。
