Qi Lu-Ming, Zhang Ji, Zhao Yan-Li, Zuo Zhi-Tian, Jin Hang, Wang Yuan-Zhong
College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China.
J Anal Methods Chem. 2017;2017:3194146. doi: 10.1155/2017/3194146. Epub 2017 Jun 1.
Franch (Gentianaceae) is a famous medicinal plant for treatments of rheumatism, convulsion, and jaundice. Comprehensive investigation of different parts and cultivation years of this plant has not yet been conducted. This study presents the quantitative and qualitative characterization of iridoid glycosides from performed by HPLC and FTIR spectroscopy techniques. The accumulations of loganic acid, swertiamarin, gentiopicroside, and sweroside were determined. Results indicated that their content and distribution in different parts and cultivation years exhibit great variations. Gentiopicroside was identified as the most abundant compound among iridoid glycosides and its highest level was observed in the root of 2-year-old plant. With respect to qualitative variation of metabolic profile, the 1800-800 cm band of FTIR spectra successfully discriminated different parts and cultivation years with the aid of PLS-DA. In addition, combined with PLSR, the feasibility of FTIR spectroscopy for determination of gentiopicroside was investigated by selecting characteristic wavelengths (1800-800 cm), which presented a good performance with a residual predictive deviation (RPD) of 3.646. Our results suggested that HPLC and FTIR techniques can complement each other and could be simultaneously applied for comparing and analyzing different parts and cultivation years of .
獐牙菜属（龙胆科）是一种著名的药用植物，可用于治疗风湿病、惊厥和黄疸。尚未对该植物的不同部位和种植年份进行全面研究。本研究通过高效液相色谱（HPLC）和傅里叶变换红外光谱（FTIR）技术对獐牙菜属植物中环烯醚萜苷进行了定量和定性表征。测定了马钱子酸、獐牙菜苦苷、龙胆苦苷和獐牙菜苷的积累量。结果表明，它们在不同部位和种植年份的含量和分布存在很大差异。龙胆苦苷被确定为环烯醚萜苷中含量最丰富的化合物，在2年生植株的根部含量最高。关于代谢谱的定性变化，借助偏最小二乘判别分析（PLS-DA），FTIR光谱的1800 - 800 cm波段成功区分了不同部位和种植年份。此外，结合偏最小二乘回归（PLSR），通过选择特征波长（1800 - 800 cm）研究了FTIR光谱法测定龙胆苦苷的可行性，其表现良好，剩余预测偏差（RPD）为3.646。我们的结果表明，HPLC和FTIR技术可以相互补充，可同时用于比较和分析獐牙菜属植物的不同部位和种植年份。
