Gou Xinlei, Gao Xia, Hu Guanghui, Chi Haitao, Le Shengfeng, Wang Wei, Liu Weili
Se Pu. 2014 Sep;32(9):988-91. doi: 10.3724/sp.j.1123.2014.06008.
A sensitive method was developed for the simultaneous determination of 11 bisphenols in plastic bottled drinking water by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were freeze-dried under vacuum and then dissolved with methanol. The separation was performed on a UPLC BEH C18 column (100 mm x 2.1 mm, 1.7 μm) by using 0.1% (v/v) NH3 · H2O and methanol as mobile phases with gradient elution at a flow rate of 0.2 mL/min. The electrospray ionization (ESI) source in negative ion mode was used for the analysis of the 11 bisphenols in the multiple reaction monitoring (MRM) mode. The results verified that the standard curves for the 11 bisphenols were obtained with good correlation coefficients (R2) > 0.997 in their concentration ranges. The limits of detection (LOD, S/N = 3) for the 11 bisphenols were in the range of 0.01-1.00 μg/L. The mean recoveries for the 11 bisphenols at three spiked levels (low, middle, high) were 75.3%-102.1% with the relative standard deviations of 1.5%-8.9%. Seven plastic bottled drinking water samples were tested, and no bisphenol was found. The method is accurate, simple, rapid and feasible for the simultaneous determination of bisphenols in plastic bottled drinking water.
建立了一种超高效液相色谱-串联质谱法（UPLC-MS/MS）同时测定塑料瓶装饮用水中11种双酚的灵敏方法。样品经真空冷冻干燥后用甲醇溶解。采用UPLC BEH C18柱（100 mm×2.1 mm，1.7μm）进行分离，以0.1%（v/v）氨水和甲醇为流动相，梯度洗脱，流速为0.2 mL/min。采用电喷雾电离（ESI）源负离子模式，在多反应监测（MRM）模式下分析11种双酚。结果表明，11种双酚的标准曲线在其浓度范围内具有良好的相关系数（R2）>0.997。11种双酚的检测限（LOD，S/N = 3）在0.01-1.00μg/L范围内。11种双酚在三个加标水平（低、中、高）的平均回收率为75.3%-102.1%，相对标准偏差为1.5%-8.9%。对7个塑料瓶装饮用水样品进行检测，未发现双酚。该方法用于同时测定塑料瓶装饮用水中的双酚准确、简便、快速、可行。
