Doctoral School of Exact and Natural Sciences, University of Bialystok, Ciołkowskiego 1K St., 15-245 Białystok, Poland.
Department of Analytical and Inorganic Chemistry, Faculty of Chemistry, University of Bialystok, Ciołkowskiego 1K St., 15-245 Białystok, Poland.
Molecules. 2022 Aug 5;27(15):4977. doi: 10.3390/molecules27154977.
A new, simple and sensitive method for isolating nine compounds from the bisphenol group (analogues: A, B, C, E, F, G, Cl, Z, AP) based on one-step liquid-liquid microextraction with in situ acylation followed by gas chromatography-mass spectrometry was developed and validated using influent and effluent wastewaters. The chemometric approach based on the Taguchi method was used to optimize the main conditions of simultaneous extraction and derivatization. The recoveries of the proposed procedure ranged from 85 to 122%, and the repeatability expressed by the coefficient of variation did not exceed 8%. The method's limits of detection were in the range of 0.4-64 ng/L, and the method's limits of quantification ranged from 1.3 to 194 ng/L. The developed method was used to determine the presence of the tested compounds in wastewater from a municipal wastewater treatment plant located in northeastern Poland. From this sample, eight analytes were detected. Concentrations of bisphenol A of 400 ng/L in influent and 100 ng/L in effluent were recorded, whereas other bisphenols reached 67 and 50 ng/L for influent and effluent, respectively. The removal efficiency of bisphenol analogues in the tested wastewater treatment plant ranged from 7 to approximately 88%.
开发并验证了一种新的、简单且灵敏的方法，用于从双酚组（类似物：A、B、C、E、F、G、Cl、Z、AP）中一步液液微萃取与原位酰化后，通过气相色谱-质谱法进行分离，使用进水和出水废水。基于田口法的化学计量学方法用于优化同时提取和衍生化的主要条件。所提出程序的回收率范围为 85%至 122%，变异系数表示的重复性不超过 8%。该方法的检测限范围为 0.4-64 ng/L，定量限范围为 1.3-194 ng/L。该方法用于测定位于波兰东北部的城市污水处理厂废水中测试化合物的存在。从该样品中检测到八种分析物。记录到进水的双酚 A 浓度为 400ng/L，出水的浓度为 100ng/L，而其他双酚类物质的进水和出水浓度分别达到 67 和 50ng/L。在测试的污水处理厂中，双酚类似物的去除效率范围为 7%至约 88%。
