Ding Chen, Cheng Lei, He Qiao, Zhang Hui
Wei Sheng Wu Xue Bao. 2014 Nov 4;54(11):1369-77.
Using molecular ecology methods, we screened non-hydrocarbon carbon sources suitable for growth of syntrophic hydrocarbon-degrading Syntrophus sp.
The acclimated methanogenic hexadecane-degrading consortium M82 was subcultured with dodecanedioic acid, tetradecanedioic acid, hexadecanoic acid, propionate and lactate. PCR-DGGE and qPCR were used to analyze the abundance and quantity of syntrophaceae using different carbon sources. The T-RFLP was applied to analyze archaeal community.
The consortium M82 could grow and produce methane using a variety of fatty acids that also resulted in the change in bacterial microbial community structure. Syntrophaceae bacterial stripe was obviously detected in the culture added additional dodecanedioic acid and tetradecanedioic acid. Furthermore, the results show that the logarithmic abundance of Syntrophaceae was 7.4 and 7.6 in per milliliter culture in the two enrichment cultures respectively, which were 2 - 3 units higher than these in other cultures. The archaeal community structure was mainly composed of acetoclastic methanogens Methanosaeta and hydrogenotrophic methanogens Methanoculleus in all culture.
Syntrophus sp. can use non-hydrocarbon carbon source (dodecanedioic acid and tetradecanedioic acid) as substrate to grow, which provides valuable information to isolate syntrophic hydrocarbon bacteria, and reveal the molecular mechanism of syntrophic hydrocarbon degradation.
运用分子生态学方法，筛选适合互营性烃降解嗜互营菌属生长的非烃类碳源。
将驯化的十六烷降解产甲烷菌群M82分别用十二烷二酸、十四烷二酸、十六烷酸、丙酸盐和乳酸盐进行传代培养。采用PCR-DGGE和qPCR分析使用不同碳源时互营菌科的丰度和数量。应用T-RFLP分析古菌群落。
菌群M82能够利用多种脂肪酸生长并产生甲烷，这也导致了细菌微生物群落结构的变化。在添加了十二烷二酸和十四烷二酸的培养物中明显检测到互营菌科细菌条带。此外，结果表明，在这两种富集培养物中，互营菌科每毫升培养物中的对数丰度分别为7.4和7.6，比其他培养物中的高2 - 3个单位。在所有培养物中，古菌群落结构主要由乙酸营养型产甲烷菌甲烷鬃菌属和氢营养型产甲烷菌甲烷袋状菌属组成。
嗜互营菌属能够利用非烃类碳源（十二烷二酸和十四烷二酸）作为底物生长，这为分离互营性烃降解细菌以及揭示互营性烃降解的分子机制提供了有价值的信息。
