Luo Jinxue, Zhang Jinsong, Barnes Robert J, Tan Xiaohui, McDougald Diane, Fane Anthony G, Zhuang Guoqiang, Kjelleberg Staffan, Cohen Yehuda, Rice Scott A
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China; School of Biological Sciences, 60 Nanyang Drive, SBS-01N-27, Singapore, 637551; Advanced Environmental Biotechnology Centre, Nanyang Technological University, Singapore.
Microb Biotechnol. 2015 May;8(3):549-60. doi: 10.1111/1751-7915.12261. Epub 2015 Mar 6.
A novel strategy to control membrane bioreactor (MBR) biofouling using the nitric oxide (NO) donor compound PROLI NONOate was examined. When the biofilm was pre-established on membranes at transmembrane pressure (TMP) of 88-90 kPa, backwashing of the membrane module with 80 μM PROLI NONOate for 45 min once daily for 37 days reduced the fouling resistance (Rf ) by 56%. Similarly, a daily, 1 h exposure of the membrane to 80 μM PROLI NONOate from the commencement of MBR operation for 85 days resulted in reduction of the TMP and Rf by 32.3% and 28.2%. The microbial community in the control MBR was observed to change from days 71 to 85, which correlates with the rapid TMP increase. Interestingly, NO-treated biofilms at 85 days had a higher similarity with the control biofilms at 71 days relative to the control biofilms at 85 days, indicating that the NO treatment delayed the development of biofilm bacterial community. Despite this difference, sequence analysis indicated that NO treatment did not result in a significant shift in the dominant fouling species. Confocal microscopy revealed that the biomass of biopolymers and microorganisms in biofilms were all reduced on the PROLI NONOate-treated membranes, where there were reductions of 37.7% for proteins and 66.7% for microbial cells, which correlates with the reduction in TMP. These results suggest that NO treatment could be a promising strategy to control biofouling in MBRs.
研究了一种使用一氧化氮（NO）供体化合物脯氨酸亚硝酸盐来控制膜生物反应器（MBR）生物污染的新策略。当在跨膜压力（TMP）为88 - 90kPa的条件下在膜上预先形成生物膜后，每天用80μM脯氨酸亚硝酸盐对膜组件进行一次45分钟的反冲洗，持续37天，污垢热阻（Rf）降低了56%。同样，从MBR运行开始，每天让膜暴露于80μM脯氨酸亚硝酸盐1小时，持续85天，导致TMP和Rf分别降低了32.3%和28.2%。观察到对照MBR中的微生物群落从第71天到第85天发生了变化，这与TMP的快速增加相关。有趣的是，相对于85天的对照生物膜，85天经NO处理的生物膜与71天的对照生物膜具有更高的相似性，这表明NO处理延缓了生物膜细菌群落的发展。尽管存在这种差异，但序列分析表明NO处理并未导致主要污垢物种发生显著变化。共聚焦显微镜显示，脯氨酸亚硝酸盐处理过的膜上生物膜中生物聚合物和微生物的生物量均有所减少，其中蛋白质减少了37.7%，微生物细胞减少了66.7%，这与TMP的降低相关。这些结果表明，NO处理可能是控制MBR中生物污染的一种有前景的策略。
