Foster M Lauren, Dowd Scot E, Stephenson Christine, Steiner Jörg M, Suchodolski Jan S
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
Vet Med Int. 2013;2013:658373. doi: 10.1155/2013/658373. Epub 2013 Apr 23.
The prevalence and phylogenetic description of fungal organisms and their role as part of the intestinal ecosystem have not yet been studied extensively in dogs. This study evaluated the fungal microbiome of 19 dogs (12 healthy dogs and 7 dogs with acute diarrhea) using fungal tag-encoded FLX-Titanium amplicon pyrosequencing. Five distinct fungal phyla were identified, with Ascomycota (medians: 97.9% of obtained sequences in healthy dogs and 98.2% in diseased dogs) and Basidiomycota (median 1.0% in healthy dogs and median 0.5% in diseased dogs) being the most abundant fungal phyla. A total of 219 fungal genera were identified across all 19 dogs with a median (range) of 28 (4-69) genera per sample. Candida was the most abundant genus found in both the diseased dogs (median: 1.9%, range: 0.2%-38.5% of sequences) and healthy dogs (median: 5.2%, range: 0.0%-63.1% of sequences). Candida natalensis was the most frequently identified species. No significant differences were observed in the relative proportions of fungal communities between healthy and diseased dogs. In conclusion, fecal samples of healthy dogs and dogs with acute diarrhea harbor various fungal genera, and their role in gastrointestinal health and disease warrants further studies.
真菌生物体在犬类中的流行情况、系统发育描述及其作为肠道生态系统一部分的作用尚未得到广泛研究。本研究使用真菌标签编码的FLX-Titanium扩增子焦磷酸测序技术，对19只犬（12只健康犬和7只急性腹泻犬）的真菌微生物群进行了评估。鉴定出了五个不同的真菌门，其中子囊菌门（中位数：健康犬中获得序列的97.9%，患病犬中为98.2%）和担子菌门（健康犬中位数为1.0%，患病犬中位数为0.5%）是最丰富的真菌门。在所有19只犬中总共鉴定出219个真菌属，每个样本的中位数（范围）为28（4-69）个属。念珠菌是在患病犬（中位数：1.9%，序列范围：0.2%-38.5%）和健康犬（中位数：5.2%，序列范围：0.0%-63.1%）中发现的最丰富的属。纳塔尔念珠菌是最常鉴定出的物种。在健康犬和患病犬的真菌群落相对比例上未观察到显著差异。总之，健康犬和急性腹泻犬的粪便样本中含有各种真菌属，它们在胃肠道健康和疾病中的作用值得进一步研究。
