School of Medicine, The University of Notre Dame Australia, Fremantle, Australia; Clinical Research Centre, North Metropolitan Health Service - Mental Health, Perth, Australia.
Research Centre for the Neurosciences of Ageing, Academic Unit of Psychiatry and Addiction Medicine, Australian National University Medical School, Canberra Hospital, Canberra, Australia.
Psychiatry Res. 2015 Apr 30;232(1):98-105. doi: 10.1016/j.pscychresns.2015.02.001. Epub 2015 Feb 12.
We present a validated protocol for manual segmentation of the thalamus on T1-weighted magnetic resonance imaging (MRI) scans using brain image analysis software. The MRI scans of five normal control subjects were randomly selected from a larger cohort recruited from Lund University Hospital and Landskrona Hospital, Sweden. MRIs were performed using a 3.0T Philips MR scanner, with an eight-channel head coil, and high resolution images were acquired using a T1-weighted turbo field echo (T1 TFE) pulse sequence, with resulting voxel size 1×1×1 mm3. Manual segmentation of the left and right thalami and volume measurement was performed on 28-30 contiguous coronal slices, using ANALYZE 11.0 software. Reliability of image analysis was performed by measuring intra-class correlations between initial segmentation and random repeated segmentation of the left and right thalami (in total 10 thalami for segmentation); inter-rater reliability was measured using volumes obtained by two other experienced tracers. Intra-class correlations for two independent raters were 0.95 and 0.98; inter-class correlations between the expert rater and two independent raters were 0.92 and 0.98. We anticipate that mapping thalamic morphology in various neuropsychiatric disorders may yield clinically useful disease-specific biomarkers.
我们提出了一种经过验证的方案，用于使用脑图像分析软件在 T1 加权磁共振成像 (MRI) 扫描上手动分割丘脑。从瑞典隆德大学医院和兰斯克鲁纳医院招募的更大队列中随机选择了五名正常对照受试者的 MRI 扫描。MRI 使用 3.0T 飞利浦 MR 扫描仪进行，配备八通道头部线圈，并使用 T1 加权涡轮场回波 (T1 TFE) 脉冲序列采集高分辨率图像，体素大小为 1×1×1mm3。使用 ANALYZE 11.0 软件在 28-30 个连续的冠状切片上对左、右丘脑进行手动分割和体积测量。通过测量左、右丘脑初始分割和随机重复分割（共 10 个丘脑进行分割）之间的组内相关系数来评估图像分析的可靠性；使用另外两名经验丰富的追踪者获得的体积来测量观察者间可靠性。两名独立观察者的组内相关系数为 0.95 和 0.98；专家观察者与两名独立观察者之间的组间相关系数为 0.92 和 0.98。我们预计，在各种神经精神障碍中绘制丘脑形态可能会产生具有临床应用价值的疾病特异性生物标志物。
