Lee Sun-Young, Moon Hee-Won, Hur Mina, Yun Yeo-Min
Department of Internal Medicine, Konkuk University School of Medicine, Seoul, Korea.
Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Korea.
J Med Microbiol. 2015 May;64(Pt 5):513-518. doi: 10.1099/jmm.0.000050. Epub 2015 Mar 9.
Helicobacter pylori infection is endemic in Korea, and serology testing is widely performed. The aim of this study was to validate and compare the diagnostic accuracy of Korean and Western serological assays for H. pylori detection in Korean adults. The 114 Korean adults who visited our centre over a 6-month period for the evaluation of H. pylori infection using the urea breath test (UBT) were enrolled in this prospective study. Anti-H. pylori IgG was measured using three commercially available immunoassays: Genedia H. pylori ELISA (Green Cross Medical Science), Chorus helicobacter IgG (DIESSE Diagnostica Senese) and Vidas H. pylori IgG (bioMérieux). Positive UBT findings were obtained in 40.6% of included subjects. The sensitivities and the specificities of Vidas, Chorus and Genedia were 89.7%, 100% and 100% and 85.5%, 75.4% and 80.7%, respectively. We found no differences in sensitivity between the Vidas and Chorus (P=0.125), Chorus and Genedia (P=0.125) and Vidas and Genedia (P=1.000) assays. There were also no differences in specificity between the Vidas and Chorus (P=0.070), Chorus and Genedia (P=0.508) and Vidas and Genedia (P=0.549) assays. In Korean adults, the Genedia H. pylori ELISA, Chorus helicobacter IgG and Vidas H. pylori IgG assays exhibited a high concurrence rate with similar diagnostic accuracy. Thus, both the Korean and Western non-invasive assays are reliable for serodiagnosis of H. pylori in Korean individuals.
幽门螺杆菌感染在韩国呈地方性流行，血清学检测广泛开展。本研究旨在验证并比较韩国和西方血清学检测方法对韩国成年人幽门螺杆菌检测的诊断准确性。在为期6个月的时间里，114名因评估幽门螺杆菌感染而到我们中心就诊并接受尿素呼气试验（UBT）的韩国成年人被纳入这项前瞻性研究。使用三种市售免疫测定法检测抗幽门螺杆菌IgG：Genedia幽门螺杆菌酶联免疫吸附测定（绿十字医药品株式会社）、Chorus幽门螺杆菌IgG（迪赛诊断试剂公司）和Vidas幽门螺杆菌IgG（生物梅里埃公司）。40.6%的纳入受试者尿素呼气试验结果呈阳性。Vidas、Chorus和Genedia的灵敏度分别为89.7%、100%和100%，特异性分别为85.5%、75.4%和80.7%。我们发现Vidas与Chorus（P = 0.125）、Chorus与Genedia（P = 0.125）以及Vidas与Genedia（P = 1.000）检测方法在灵敏度上无差异。Vidas与Chorus（P = 0.070）、Chorus与Genedia（P = 0.508）以及Vidas与Genedia（P = 0.549）检测方法在特异性上也无差异。在韩国成年人中，Genedia幽门螺杆菌酶联免疫吸附测定、Chorus幽门螺杆菌IgG和Vidas幽门螺杆菌IgG检测方法显示出较高的一致性，诊断准确性相似。因此，韩国和西方的非侵入性检测方法对韩国个体幽门螺杆菌的血清学诊断都是可靠的。
