Nagashima Y, Ohaki Y, Umeda M, Oshimura M, Misugi K
Department of Pathology, Yokohama City University, School of Medicine, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;57(6):383-92. doi: 10.1007/BF02899105.
A new cell line designated ENU-T-1 has been established from a xenotransplanted experimental rat nephroblastoma. The cultured cells are spindle-shaped or polygonal and are arranged in a wavy fashion morphologically similar to cultured embryonal renal epithelial cells. The cells exhibit a number of epithelial characteristics. Enzyme histochemistry gives positive reactions for gamma-glutamyltranspeptidase and alkaline phosphatase, both of which are present in renal tubular epithelial cells. Immunofluorescence studies show positive reactions for vimentin and cytokeratin. When inoculated into athymic nude mice, the cultured cells form tumors composed of sheets of epithelial cells with focal tubular formation. This cell line may be of value in studying differentiation of nephroblastoma, and possibly normal nephrogenesis.
一种名为ENU-T-1的新细胞系已从异种移植的实验性大鼠肾母细胞瘤中建立。培养的细胞呈纺锤形或多边形，以波浪状排列，形态上类似于培养的胚胎肾上皮细胞。这些细胞表现出许多上皮细胞特征。酶组织化学显示γ-谷氨酰转肽酶和碱性磷酸酶呈阳性反应，这两种酶都存在于肾小管上皮细胞中。免疫荧光研究显示波形蛋白和细胞角蛋白呈阳性反应。当接种到无胸腺裸鼠体内时，培养的细胞形成由成片上皮细胞组成的肿瘤，并伴有局灶性管状结构。该细胞系在研究肾母细胞瘤的分化以及可能的正常肾发生方面可能具有价值。
