Shibuya K, Kizaki H, Tajima M, Shibuya N, Yamate J, Nunoya T
Nippon Institute for Biological Science, Tokyo, Japan.
J Vet Med Sci. 1995 Dec;57(6):1117-20. doi: 10.1292/jvms.57.1117.
A new cell line, designated NB-YK, was established from a transplantable rat nephroblastoma (NB-Y) which was derived from a spontaneous nephroblastoma in an aged Fischer 344 rat. NB-YK grew in a piling-up and noncohesive pattern on the plastic surface and formed colonies in a soft agar. The main cell type of NB-YK represented morphology of mesenchymal phenotype and most of the cells contained several secretory granules in their cytoplasm. Immunocytochemically the cells were positive for vimentin, cytokeratin, and laminin. Coexpression of vimentin and cytokeratin in the cells was confirmed by the one-dimensional gel electrophoresis and immunoblotting for intermediate filament proteins. NB-YK cells were tumorigenic and produced fibrosarcoma-like tumor when inoculated subcutaneously or intraperitoneally into syngeneic rats and nude mice. NB-YK seems to be a useful model for studying biological properties of nephroblastoma.
一种名为NB - YK的新细胞系，是从一只可移植的大鼠肾母细胞瘤（NB - Y）中建立的，该肾母细胞瘤源自一只老年Fischer 344大鼠的自发性肾母细胞瘤。NB - YK在塑料表面以堆积且不粘连的模式生长，并在软琼脂中形成集落。NB - YK的主要细胞类型表现出间充质表型的形态，大多数细胞的细胞质中含有多个分泌颗粒。免疫细胞化学检测显示，这些细胞波形蛋白、细胞角蛋白和层粘连蛋白呈阳性。通过一维凝胶电泳和中间丝蛋白免疫印迹法证实了细胞中波形蛋白和细胞角蛋白的共表达。将NB - YK细胞皮下或腹腔内接种到同基因大鼠和裸鼠体内时具有致瘤性，并产生纤维肉瘤样肿瘤。NB - YK似乎是研究肾母细胞瘤生物学特性的有用模型。
