Momii S, Koga A, Eguchi M, Fukuyama T
Department of Surgery I, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;57(6):393-8. doi: 10.1007/BF02899106.
Changes in the sinusoids of rat livers stored in cold (2 degrees C) Euro-Collins solution for various periods were observed using combined scanning (SEM) and transmission electron microscopy (TEM). The sinusoidal endothelial cells were vulnerable to cold ischemia. Fenestrations of the endothelial cells were enlarged and became mesh-like after a 4-h preservation period. Following 8 h storage the sieve plates and cytoplasmic processes of the endothelial cells were destroyed and there was a tendency for the perikaryon to desquamate. Blebs derived from hepatocytes were seen after 4 h and these increased in number and size with prolonged preservation. Although the sinusoids were filled with blebs after 24 h preservation. no irreversible ultrastructural damage in the parenchymal cells was observed. Within 12 h storage, the liver had a mosaic pattern after perfusion fixation indicating uneven fixation and profound circulatory disturbance. These results suggest that endothelial cell destruction and/or numerous blebs may have unfavorable effects on the microcirculation of the transplanted liver after prolonged preservation.
运用扫描电子显微镜（SEM）和透射电子显微镜（TEM）相结合的方法，观察了大鼠肝脏在冷（2℃）Euro - Collins溶液中保存不同时间后肝血窦的变化。肝血窦内皮细胞对冷缺血敏感。保存4小时后，内皮细胞的窗孔扩大并呈网状。保存8小时后，内皮细胞的筛板和细胞质突起被破坏，核周有脱落倾向。4小时后可见源自肝细胞的小泡，随着保存时间延长，其数量和大小增加。保存24小时后，肝血窦充满小泡，但实质细胞未观察到不可逆的超微结构损伤。保存12小时内，灌注固定后肝脏呈镶嵌模式，表明固定不均匀且存在严重的循环障碍。这些结果表明，长时间保存后，内皮细胞破坏和/或大量小泡可能对移植肝脏的微循环产生不利影响。
