Myagkaya G L, van Veen H A, James J
Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;53(3):176-82. doi: 10.1007/BF02890241.
The ultrastructural alterations in liver tissue induced by in vitro ischemia at 4 degrees C under conditions commonly used for transplantation (Euro-Collins perfused and stored liver tissue) have been compared with changes due to hypothermic in vitro ischemia in non-perfused liver. It was found that the process of cell deterioration in non-perfused liver occurred very slowly; signs of irreversible damage appeared in sinusoidal lining cells before hepatocytes (after 24 and 96 h, respectively). Liver perfused with, and stored in Euro-Collins solution showed acceleration of the ischemical damage in both types of cell (irreversible damage to sinusoidal lining cells after 12 h and to hepatocytes after 52 h), compared with non-perfused liver. These findings indicate that the safe period for storage of rat liver in Euro-Collins before damage to the microcirculatory system is less than 12 h. It might also be questioned whether Euro-Collins treatment is the optimal procedure for tissue preservation before liver transplantation.
在常用于移植的条件下（用欧洲柯林斯液灌注并储存的肝组织），将4℃体外缺血诱导的肝组织超微结构改变与非灌注肝低温体外缺血引起的变化进行了比较。结果发现，非灌注肝中的细胞恶化过程非常缓慢；不可逆损伤的迹象在肝实质细胞之前出现在窦状隙衬里细胞中（分别在24小时和96小时后）。与非灌注肝相比，用欧洲柯林斯液灌注并储存的肝在两种类型的细胞中均显示出缺血损伤加速（窦状隙衬里细胞在12小时后出现不可逆损伤，肝实质细胞在52小时后出现不可逆损伤）。这些发现表明，在大鼠肝微循环系统受损之前，用欧洲柯林斯液储存的安全时间少于12小时。欧洲柯林斯液处理是否是肝移植前组织保存的最佳方法也可能受到质疑。
