Yokota Yoko, Nishiyama Norihiro
Nihon University Graduate School of Dentistry at Matsudo; Department of Renascent Dentistry, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho, Nishi 2, Chiba 271-8587, Japan.
Dent Mater J. 2015;34(2):270-9. doi: 10.4012/dmj.2014-260. Epub 2015 Mar 4.
Enamel and dentin particles were added to an experimental 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-based one-step adhesive to react for 30 s. After enamel and dentin reactants were analyzed using X-ray diffraction (XRD) and phosphorus-31 nuclear magnetic resonance ((31)P NMR) techniques, curve-fitting analysis was performed on the (31)P NMR spectra of enamel and dentin reactants. By varying the molar ratio of calcium chloride to MDP, a series of three types of MDP-Ca salts were synthesized. The molecular species of calcium salts of MDP (MDP-Ca salts) produced by decalcification of enamel or dentin were determined based on the XRD and (31)P NMR analysis results of these three types of synthesized MDP-Ca salts. Curve-fitting analysis showed that enamel and dentin developed several types of MDP-Ca salts and amorphous dicalcium phosphate dihydrate (DCPD) during decalcification. The molecular species of MDP-Ca salts produced by enamel and dentin were mono-calcium salts of MDP monomer and MDP dimer. In addition, dentin produced a di-calcium of MDP dimer.
将牙釉质和牙本质颗粒添加到基于实验性10-甲基丙烯酰氧基癸基磷酸二氢酯（MDP）的单步粘合剂中反应30秒。使用X射线衍射（XRD）和磷-31核磁共振（³¹P NMR）技术分析牙釉质和牙本质反应物后，对牙釉质和牙本质反应物的³¹P NMR光谱进行曲线拟合分析。通过改变氯化钙与MDP的摩尔比，合成了一系列三种类型的MDP-Ca盐。基于这三种合成的MDP-Ca盐的XRD和³¹P NMR分析结果，确定了由牙釉质或牙本质脱钙产生的MDP钙盐（MDP-Ca盐）的分子种类。曲线拟合分析表明，牙釉质和牙本质在脱钙过程中形成了几种类型的MDP-Ca盐和无定形二水磷酸二钙（DCPD）。牙釉质和牙本质产生的MDP-Ca盐的分子种类是MDP单体和MDP二聚体的单钙盐。此外，牙本质产生了MDP二聚体的二钙盐。
