Lewandowski Eric M, Skiba Joanna, Torelli Nicholas J, Rajnisz Aleksandra, Solecka Jolanta, Kowalski Konrad, Chen Yu
Department of Molecular Medicine, University of South Florida, Tampa, Florida 33612, USA.
Chem Commun (Camb). 2015 Apr 11;51(28):6186-9. doi: 10.1039/c5cc00904a.
We have determined a 1.18 Å resolution X-ray crystal structure of a novel ruthenocenyle-6-aminopenicillinic acid in complex with CTX-M β-lactamase, showing unprecedented details of interactions between ruthenocene and protein. As the first product complex with an intact catalytic serine, the structure also offers insights into β-lactamase catalysis and inhibitor design.
我们已经确定了一种新型钌茂烯基-6-氨基青霉烷酸与CTX-Mβ-内酰胺酶复合物的X射线晶体结构，分辨率为1.18Å，展示了钌茂烯与蛋白质之间前所未有的相互作用细节。作为第一个与完整催化丝氨酸形成的产物复合物，该结构还为β-内酰胺酶催化和抑制剂设计提供了见解。
