Kaliniene Laura, Zajančkauskaitė Aurelija, Šimoliūnas Eugenijus, Truncaitė Lidija, Meškys Rolandas
Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Mokslininkų 12, 08662, Vilnius, Lithuania,
Arch Virol. 2015 May;160(5):1367-70. doi: 10.1007/s00705-015-2388-0. Epub 2015 Mar 10.
The complete genome sequences of four low-temperature Escherichia coli-specific tevenviruses, vb_EcoM-VR5, vb_EcoM-VR20, vb_EcoM-VR25 and vb_EcoM-VR26, were determined. Genomic comparisons including recently described genomes of vb_EcoM-VR7 and JS98 as well as phage T4 allowed the identification of two genetic groups that were consistent with defined host-range phenotypes. Group A included the broad-host-range phages vb_EcoM-VR5 and JS98, while group B included vb_EcoM-VR7, vb_EcoM-VR20, vb_EcoM-VR25 and vb_EcoM-VR26, which all had somewhat limited host ranges. All four sequenced phages had genomes that were similar in length (170 kb) and GC content (40 %), and, with the exception of vb_EcoM-VR5, at the nucleotide level, they were much more closely related to each other than either was to any other tevenvirus currently characterized. Nevertheless, the overall genome organization of vb_EcoM-VR5, vb_EcoM-VR20, vb_EcoM-VR25 and vb_EcoM-VR26 was comparable to that seen in tevenviruses.
测定了四种低温大肠杆菌特异性T偶数病毒vb_EcoM-VR5、vb_EcoM-VR20、vb_EcoM-VR25和vb_EcoM-VR26的全基因组序列。基因组比较包括最近描述的vb_EcoM-VR7和JS98的基因组以及噬菌体T4，从而确定了两个与确定的宿主范围表型一致的基因组群。A组包括广宿主范围噬菌体vb_EcoM-VR5和JS98，而B组包括vb_EcoM-VR7、vb_EcoM-VR20、vb_EcoM-VR25和vb_EcoM-VR26，它们的宿主范围都有些有限。所有四种测序噬菌体的基因组长度（约170 kb）和GC含量（约40%）相似，并且，除vb_EcoM-VR5外，在核苷酸水平上，它们彼此之间的关系比它们与目前已鉴定的任何其他T偶数病毒的关系更为密切。然而，vb_EcoM-VR5、vb_EcoM-VR20、vb_EcoM-VR25和vb_EcoM-VR26的总体基因组组织与T偶数病毒中的情况相当。
