通过SYBR Green实时荧光定量PCR鉴别溶血嗜血杆菌和流感嗜血杆菌。
Identifying Haemophilus haemolyticus and Haemophilus influenzae by SYBR Green real-time PCR.
作者信息
Latham Roger, Zhang Bowen, Tristram Stephen
机构信息
School of Medicine, University of Tasmania, Hobart, Tasmania, Australia.
School of Health Sciences, University of Tasmania, Launceston, Australia.
出版信息
J Microbiol Methods. 2015 May;112:67-9. doi: 10.1016/j.mimet.2015.03.001. Epub 2015 Mar 6.
PMID:25753676
Abstract
SYBR Green real time PCR assays for protein D (hpd), fuculose kinase (fucK) and [Cu, Zn]-superoxide dismutase (sodC) were designed for use in an algorithm for the identification of Haemophilus influenzae and H. haemolyticus. When tested on 127 H. influenzae and 60 H. haemolyticus all isolates were identified correctly.
摘要
设计了用于检测蛋白质D（hpd）、岩藻糖激酶（fucK）和[铜，锌]超氧化物歧化酶（sodC）的SYBR Green实时PCR检测方法，用于流感嗜血杆菌和溶血嗜血杆菌鉴定算法。在127株流感嗜血杆菌和60株溶血嗜血杆菌上进行测试时，所有分离株均被正确鉴定。
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