硫胺素和硫胺素焦磷酸对大鼠酒精性肝损伤生物标志物的影响。
The effects of thiamine and thiamine pyrophosphate on alcohol-induced hepatic damage biomarkers in rats.
作者信息
Yilmaz I, Demiryilmaz I, Turan M I, Çetin N, Gul M A, Süleyman H
机构信息
Department of Surgery, Erzincan University, Faculty of Medicine, Erzincan, Turkey.
出版信息
Eur Rev Med Pharmacol Sci. 2015 Feb;19(4):664-70.
OBJECTIVE
This study examined the effects of thiamine and thiamine pyrophosphate on oxidative damage developing in association with hepatic injury caused by alcohol toxicity in rats and on hepatic injury markers.
MATERIALS AND METHODS
Four groups of rats were used; control, a group receiving thiamine+ethanol, a group receiving thiamine pyrophosphate+ethanol and a healthy group. The experimental protocol was repeated over 30 days. Malondialdehyde, glutathione and DNA damage product levels in liver tissue were measured at the end of the study. Alanine amino transferase and aspartate amino transferase, markers of liver damage, levels were determined. The results were then compared among the groups.
RESULTS
A statistically significant difference between antioxidant markers and markers of liver damage was determined between the group given thiamine pyrophosphate ethanol and the group given ethanol alone (p < 0.01) No statistically significant difference was observed between the group given thiamine and ethanol and the group given ethanol alone (p > 0.01).
CONCLUSIONS
Our results suggest that thiamine pyrophosphate may have a protective effect against liver damage caused by alcohol toxicity.
目的
本研究考察了硫胺素和硫胺素焦磷酸对大鼠酒精毒性所致肝损伤相关氧化损伤的影响以及对肝损伤标志物的影响。
材料与方法
使用四组大鼠；对照组、接受硫胺素+乙醇的组、接受硫胺素焦磷酸+乙醇的组以及健康组。实验方案重复30天。在研究结束时测量肝组织中的丙二醛、谷胱甘肽和DNA损伤产物水平。测定肝损伤标志物丙氨酸氨基转移酶和天冬氨酸氨基转移酶的水平。然后对各实验组的结果进行比较。
结果
在给予硫胺素焦磷酸乙醇的组和仅给予乙醇的组之间，抗氧化标志物和肝损伤标志物存在统计学上的显著差异（p<0.01）。在给予硫胺素和乙醇的组与仅给予乙醇的组之间未观察到统计学上的显著差异（p>0.01）。
结论
我们的结果表明，硫胺素焦磷酸可能对酒精毒性所致肝损伤具有保护作用。
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