Abdulmajeed N A, Alnahdi H S, Ayas N O, Mohamed A M
Biochemistry Department, Faculty of Science for Girls, King Abdulaziz University, Jeddah, Kingdon of Saudi Arabia.
Eur Rev Med Pharmacol Sci. 2015 Feb;19(4):671-81.
The safety of Non-steroidal anti-inflammatory drugs (NSAIDs) use in clinical practice has been questioned. Clinical studies indicate that these drugs cause adverse cardiovascular effects. The aim of this study was to investigate the protective role of vitamin B complex against the cardiotoxic potency of diclofenac sodium induced cardiac damage.
Diclofenac sodium was administered intraperitoneally to rats at either 1.5 mg or 3 mg/kg body weight for 14 consecutive days. Vitamin B complex (1.6 mg B1, 1.6 mg B6 and 16.7 µg B12/kg body weight, i.p.) was co-administered daily for 3 weeks along with diclofenac administration to rats intoxicated by either of the two doses.
The results revealed that co-administration of vitamin B complex with diclofenac to rats intoxicated by either of the two doses, markedly ameliorated increases in serum markers of cardiac damage, including, (AST), creatine kinase-MB (CK-MB) as well as decreases in phosphoglucoisomerase (PGI) and lactate dehydrogenase (LDH) activities in cardiac tissue compared with intoxicated rats. The B complex also could markedly attenuate the decreases in cardiac antioxidant enzymes namely, glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G-6-PDH) and catalase (CAT) compared with diclofenac intoxicated rats. Beside, the vitamin B complex successfully modulated the increases in serum glucose, serum lipid profiles, triglycerides (TGs), total cholesterol (TCh) and low density lipoprotein (LDL-C) as well as the decrease in the high density lipoprotein (HDL-C) in response to diclofenac toxicity.
These results support the use of vitamin B complex along with diclofenac therapy as a protective agent against cardiac tissue damage induced by diclofenac toxicity.
非甾体抗炎药（NSAIDs）在临床实践中的安全性受到质疑。临床研究表明，这些药物会引起不良心血管效应。本研究的目的是调查复合维生素B对双氯芬酸钠所致心脏毒性及心脏损伤的保护作用。
将双氯芬酸钠以1.5毫克或3毫克/千克体重的剂量连续14天腹腔注射给大鼠。复合维生素B（1.6毫克维生素B1、1.6毫克维生素B6和16.7微克维生素B12/千克体重，腹腔注射）与双氯芬酸钠同时给药，持续3周，用于两种剂量中毒的大鼠。
结果显示，将复合维生素B与双氯芬酸钠同时给予两种剂量中毒的大鼠，与中毒大鼠相比，显著改善了心脏损伤血清标志物的升高，包括天冬氨酸转氨酶（AST）、肌酸激酶同工酶（CK-MB），以及心脏组织中磷酸葡萄糖异构酶（PGI）和乳酸脱氢酶（LDH）活性的降低。与双氯芬酸钠中毒大鼠相比，复合维生素B还可显著减轻心脏抗氧化酶即谷胱甘肽还原酶（GR）、葡萄糖-6-磷酸脱氢酶（G-6-PDH）和过氧化氢酶（CAT）的降低。此外，复合维生素B成功调节了因双氯芬酸钠毒性导致的血清葡萄糖、血脂谱、甘油三酯（TGs）、总胆固醇（TCh）和低密度脂蛋白（LDL-C）的升高，以及高密度脂蛋白（HDL-C）的降低。
这些结果支持在双氯芬酸钠治疗中使用复合维生素B作为预防双氯芬酸钠毒性所致心脏组织损伤的保护剂。
