Yao Yao, Sui Xin Hua, Zhang Xiao Xia, Wang En Tao, Chen Wen Xn
State Key Lab for Agro-Biotechnology; Ministry of Agriculture Key Lab of Soil Microbiology; College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2015 Jun;65(Pt 6):1831-1837. doi: 10.1099/ijs.0.000183. Epub 2015 Mar 9.
Six slow-growing rhizobial strains isolated from effective nodules of Erythrophleum fordii were classified into the genus Bradyrhizobiumbased on their 16S rRNA gene sequences. The results of multilocus sequence analysis of recA, glnII and gyrB genes and 16S-23S rRNA intergenic spacer (IGS) sequence phylogeny indicated that the six strains belonged to two novel species, represented by CCBAU 53325T and CCBAU 51502T, which were consistent with the results of DNA-DNA hybridization; CCBAU 53325T had 17.65-25.59 % relatedness and CCBAU 51502T had 22.69-44.58 % relatedness with five closely related type strains, Bradyrhizobium elkanii USDA 76T, B. pachyrhizi LMG 24246T, B. lablabi CCBAU 23086T, B. jicamae LMG 24556T and B. japonicum USDA 6T. In addition, analysis of phenotypic characteristics and fatty acid profiles also distinguished the test strains from defined species of Bradyrhizobium. Two novel species, Bradyrhizobium erythrophlei sp. nov., represented by the type strain CCBAU 53325T ( = HAMBI 3614T = CGMCC 1.13002T = LMG 28425T), and Bradyrhizobium ferriligni sp. nov., represented by the type strain CCBAU 51502T ( = HAMBI 3613T = CGMCC 1.13001T), are proposed to accommodate the strains.
从厚荚相思有效根瘤中分离出的6株慢生根瘤菌菌株，基于其16S rRNA基因序列被归类为慢生根瘤菌属。recA、glnII和gyrB基因的多位点序列分析结果以及16S-23S rRNA基因间隔区（IGS）序列系统发育分析表明，这6株菌株属于两个新物种，分别以CCBAU 53325T和CCBAU 51502T为代表，这与DNA-DNA杂交结果一致；CCBAU 53325T与5株密切相关的模式菌株，即埃氏慢生根瘤菌USDA 76T、大豆慢生根瘤菌LMG 24246T、扁豆慢生根瘤菌CCBAU 23086T、豆薯慢生根瘤菌LMG 24556T和日本慢生根瘤菌USDA 6T的亲缘关系为17.65-25.59%，CCBAU 51502T与它们的亲缘关系为22.69-44.58%。此外，表型特征和脂肪酸谱分析也将测试菌株与已定义的慢生根瘤菌物种区分开来。提议将这两个新物种分别命名为费氏慢生根瘤菌（Bradyrhizobium erythrophlei sp. nov.），以模式菌株CCBAU 53325T（=HAMBI 3614T = CGMCC 1.13002T = LMG 28425T）为代表；和铁木慢生根瘤菌（Bradyrhizobium ferriligni sp. nov.），以模式菌株CCBAU 51502T（=HAMBI 3613T = CGMCC 1.13001T）为代表，以容纳这些菌株。
