红茶浸液对黄曲霉毒素诱导的小鼠肝毒性的保护作用。
Protective effect of black tea infusion on aflatoxin-induced hepatotoxicity in mice.
作者信息
Jha Anamika, Krithika Rajesh, Manjeet Dave, Verma Ramtej J
机构信息
Department of Biochemistry, St. Pious X PG College for Women, Nacharam, Hyderabad 500076, India.
Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad 380009, India.
出版信息
J Clin Exp Hepatol. 2013 Mar;3(1):29-36. doi: 10.1016/j.jceh.2012.12.003. Epub 2012 Dec 31.
BACKGROUND
Aflatoxins are a group of mycotoxins produced by Aspergillus flavus and Aspergillus parasiticus and are potent inducers of hepatotoxicity.
OBJECTIVE
The present study was carried out to investigate the effect of black tea infusion on aflatoxin-induced hepatotoxicity in male mice.
METHODS
A 2% black tea infusion in drinking water was prepared and orally administered along with aflatoxin (750 and 1500 μg/kg body weight) for 30 days. Morphological investigation, body weight and organ weight calculations and histopathological analysis were carried out. Serum hepatic marker enzymes namely alanine aminotransferase and aspartate aminotransferase were estimated.
RESULTS
The results clearly indicated that aflatoxin treatment for 30 days caused significant dose-dependent reduction in body weight and increase in liver weight. The activities of ALT and AST were found to be elevated while protein content was found to be decreased in aflatoxin-treated mice as compared to vehicle control. Histopathological analysis showed hepatocellular necrosis and cytoplasmic vacuolization along with fatty infiltration in toxin-treated animals. Results revealed significant (p < 0.05) restoration of aflatoxin-induced damages in body weight, organ weight, serum chemistry and histopathological features in aflatoxin plus black tea infusion administered mice in a dose dependant manner.
CONCLUSION
It is concluded from the present study that supplementation of black tea infusion can be beneficial in positively modulating aflatoxin-induced alterations in liver.
背景
黄曲霉毒素是由黄曲霉和寄生曲霉产生的一组霉菌毒素，是肝毒性的强效诱导剂。
目的
本研究旨在探讨红茶浸液对雄性小鼠黄曲霉毒素诱导的肝毒性的影响。
方法
制备2%的红茶浸液，与黄曲霉毒素（750和1500μg/kg体重）一起口服给药30天。进行形态学研究、体重和器官重量计算以及组织病理学分析。测定血清肝标志物酶，即丙氨酸转氨酶和天冬氨酸转氨酶。
结果
结果清楚地表明，黄曲霉毒素处理30天导致体重显著剂量依赖性降低和肝脏重量增加。与溶剂对照组相比，黄曲霉毒素处理的小鼠中ALT和AST的活性升高，而蛋白质含量降低。组织病理学分析显示，毒素处理的动物出现肝细胞坏死、细胞质空泡化以及脂肪浸润。结果显示，给予黄曲霉毒素加红茶浸液的小鼠，其体重、器官重量、血清化学和组织病理学特征中黄曲霉毒素诱导的损伤以剂量依赖方式得到显著（p<0.05）恢复。
结论
从本研究得出结论，补充红茶浸液可能有利于正向调节黄曲霉毒素诱导的肝脏改变。
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