Liu Zhimin, Liu Xiaoyun, Sang Lili, Liu Haifeng, Xu Qinghua, Liu Zhehui
Department of Cadre Health Protection, Liaocheng People's Hospital Liaocheng 252000, China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):236-43. eCollection 2015.
Asthma is a serious global health problem characterised by airway inflammation, airway epithelial wall shedding, enhanced mucus production, increased IgE levels and airway hyperresponsiveness. The pathophysiology of asthma is mediated by Th2 cells which produce Th2 cytokines like interleukin-4, interleukin-5, interleukin-13 and interleukin-9. The differentiation of Th2 cells is induced by the transcription factor GATA3 which is activated by pSTAT6 via IL-4 signalling. To investigate the anti-asthmatic potential of Boswellic acid, as well as the underlying mechanism involved, we studied its anti-asthmatic potential in a murine model of asthma. In this study, BALB/c mice were systemically sensitized by ovalbumin (OVA) followed by aerosol allergen challenges. We investigated the effect of Boswellic acid on airway hyperresponsiveness, inflammatory cell infiltration, Th2 cytokine and OVA-specific IgE production in a mouse model of asthma. We found that Boswellic acid treated groups suppressed allergic airway inflammation, AHR, OVA-specific IgE and Th2 cytokines secretion. It also suppressed the expression of pSTAT6 and GATA3 in a dose dependent manner. Our data suggest that the mechanism by which Boswellic acid effectively treats asthma is based on reductions of Th2 cytokines via inhibition of pSTAT6 and GATA-3 expression.
哮喘是一个严重的全球性健康问题，其特征为气道炎症、气道上皮壁脱落、黏液分泌增加、免疫球蛋白E（IgE）水平升高以及气道高反应性。哮喘的病理生理学由产生白细胞介素-4、白细胞介素-5、白细胞介素-13和白细胞介素-9等Th2细胞因子的Th2细胞介导。Th2细胞的分化由转录因子GATA3诱导，而GATA3通过IL-4信号传导被磷酸化信号转导及转录激活因子6（pSTAT6）激活。为了研究乳香酸的抗哮喘潜力及其潜在机制，我们在哮喘小鼠模型中研究了其抗哮喘潜力。在本研究中，BALB/c小鼠经卵清蛋白（OVA）全身致敏，随后进行雾化过敏原激发。我们在哮喘小鼠模型中研究了乳香酸对气道高反应性、炎性细胞浸润、Th2细胞因子和OVA特异性IgE产生的影响。我们发现，乳香酸治疗组抑制了过敏性气道炎症、气道高反应性、OVA特异性IgE和Th2细胞因子的分泌。它还以剂量依赖的方式抑制了pSTAT6和GATA3的表达。我们的数据表明，乳香酸有效治疗哮喘的机制是通过抑制pSTAT6和GATA-3的表达来减少Th2细胞因子。
