Li Chun-Hong, Zuo Hua-Li, Chen Cen, Hu Yuan-Jia, Qian Zheng-Ming, Li Wen-Jia, Xia Zhi-Ning, Yang Feng-Qing
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
Pak J Pharm Sci. 2018 Mar;31(2):447-454.
Ganoderma lucidum (Chizhi in Chinese) is one of the most valuable and widely used medicinal fungi in traditional Chinese medicines (TCMs). Most of previous studies were focused on the triterpenoids and polysaccharides of G. lucidum, whereas less attention had been paid on the protein, which is another bioactive compound in it. In the present study, protein maps of fourteen G. lucidum samples were comprehensively analyzed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). The results indicated that there were significant differences in protein profiles of G. lucidum samples from different origins. Furthermore, previous reported bioactive proteins from the fruiting bodies of G. lucidum, were mainly distributed in 4 taxa (A, B, C and D) based on their molecular weights on the 2-DE maps. The proteins should be considered as marker for the quality control of G. lucidum, because the proteomic variation may affect on their pharmacological activities.
灵芝（中文名为赤芝）是传统中药中最具价值且应用广泛的药用真菌之一。此前的大多数研究都集中在灵芝的三萜类化合物和多糖上，而对其中的另一种生物活性化合物——蛋白质的关注较少。在本研究中，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和二维电泳（2-DE）对14个灵芝样品的蛋白质图谱进行了全面分析。结果表明，不同产地的灵芝样品蛋白质谱存在显著差异。此外，此前报道的来自灵芝子实体的生物活性蛋白，根据其在二维电泳图谱上的分子量主要分布在4个类别（A、B、C和D）中。这些蛋白质应被视为灵芝质量控制的标志物，因为蛋白质组学变异可能会影响其药理活性。
