Ribeiro Sabrina M, Bueno Paula C P, Cavalheiro Alberto José, Klein Marlise I
Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, SP, Brazil.
Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-900, SP, Brazil.
Antibiotics (Basel). 2023 Feb 4;12(2):329. doi: 10.3390/antibiotics12020329.
The effects of extracts, fractions, and molecules of to control the cariogenic biofilm of were evaluated. First, the antimicrobial and antibiofilm (initial and pre-formed biofilms) in prolonged exposure (24 h) models were investigated. Second, formulations (with and without fluoride) were assessed for topical effects (brief exposure) on biofilms. Third, selected treatments were evaluated via bacterium growth inhibition curves associated with gene expression and scanning electron microscopy. In initial biofilms, the ethyl acetate (AcOEt) and ethanolic (EtOH) fractions from Brasília (BRA/DF; 250 µg/mL) and Presidente Venceslau/SP (Water/EtOH 60:40 and Water/EtOH 40:60; 500 µg/mL) reduced ≥6-logs vs. vehicle. Only the molecule Caseargrewiin F (CsF; 125 µg/mL) reduced the viable cell count of pre-formed biofilms (5 logs vs. vehicle). For topical effects, no formulation affected biofilm components. For the growth inhibition assay, CsF yielded a constant recovery of surviving cells (≅3.5 logs) until 24 h (i.e., bacteriostatic), and AcOEt_BRA/DF caused progressive cell death, without cells at 24 h (i.e., bactericidal). CsF and AcOEt_BRA/DF damaged cells and influenced the expression of virulence genes. Thus, an effect against biofilms occurred after prolonged exposure due to the bacteriostatic and/or bactericidal capacity of a fraction and a molecule from .
评估了[植物名称]提取物、馏分和分子对控制[致龋生物膜相关细菌名称]致龋生物膜的效果。首先，研究了在长时间暴露（24小时）模型中的抗菌和抗生物膜（初始和预形成生物膜）作用。其次，评估了（含氟和不含氟）制剂对生物膜的局部作用（短暂暴露）。第三，通过与基因表达和扫描电子显微镜相关的细菌生长抑制曲线对选定的处理进行评估。在初始生物膜中，来自巴西利亚（BRA/DF；250μg/mL）的乙酸乙酯（AcOEt）和乙醇（EtOH）馏分以及来自总统文塞斯劳/圣保罗（水/乙醇60:40和水/乙醇40:60；500μg/mL）的馏分与赋形剂相比减少了≥6个对数。只有分子酪蛋白生长因子F（CsF；125μg/mL）降低了预形成生物膜中的活细胞计数（与赋形剂相比减少5个对数）。对于局部作用，没有制剂影响生物膜成分。对于生长抑制试验，CsF在24小时之前使存活细胞持续恢复（约3.5个对数）（即抑菌），而AcOEt_BRA/DF导致细胞逐渐死亡，在24小时时无细胞（即杀菌）。CsF和AcOEt_BRA/DF破坏了[细菌名称]细胞并影响了毒力基因的表达。因此，由于[植物名称]的一个馏分和一个分子的抑菌和/或杀菌能力，在长时间暴露后出现了对生物膜的作用。
