以拟南芥根系生长为例的植物全基因组关联图谱分析。
Genome-wide association mapping in plants exemplified for root growth in Arabidopsis thaliana.
作者信息
Slovak Radka, Göschl Christian, Seren Ümit, Busch Wolfgang
机构信息
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
出版信息
Methods Mol Biol. 2015;1284:343-57. doi: 10.1007/978-1-4939-2444-8_17.
Genome-wide association (GWA) mapping is a powerful technique to address the molecular basis of genotype to phenotype relationships and to map regulators of biological processes. This chapter presents a protocol for genome-wide association mapping in Arabidopsis thaliana using the user-friendly internet application GWAPP, and provides a specific protocol for acquiring root trait data suitable for GWA studies using the semi-automated, high-throughput phenotyping pipeline BRAT for early root growth.
全基因组关联（GWA）图谱分析是一种强大的技术，可用于探究基因型与表型关系的分子基础，并绘制生物过程的调控因子图谱。本章介绍了一种使用用户友好的互联网应用程序GWAPP在拟南芥中进行全基因组关联图谱分析的方案，并提供了一种特定方案，用于使用半自动化、高通量表型分析流程BRAT获取适合GWA研究的根系性状数据，以研究早期根系生长。
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