Slovak Radka, Göschl Christian, Seren Ümit, Busch Wolfgang
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
Methods Mol Biol. 2015;1284:343-57. doi: 10.1007/978-1-4939-2444-8_17.
Genome-wide association (GWA) mapping is a powerful technique to address the molecular basis of genotype to phenotype relationships and to map regulators of biological processes. This chapter presents a protocol for genome-wide association mapping in Arabidopsis thaliana using the user-friendly internet application GWAPP, and provides a specific protocol for acquiring root trait data suitable for GWA studies using the semi-automated, high-throughput phenotyping pipeline BRAT for early root growth.
全基因组关联（GWA）图谱分析是一种强大的技术，可用于探究基因型与表型关系的分子基础，并绘制生物过程的调控因子图谱。本章介绍了一种使用用户友好的互联网应用程序GWAPP在拟南芥中进行全基因组关联图谱分析的方案，并提供了一种特定方案，用于使用半自动化、高通量表型分析流程BRAT获取适合GWA研究的根系性状数据，以研究早期根系生长。
