Wang Xunming, Wang Xiaohui, Feng Yan, Zhu Manzhou, Yin Hang, Guo QingXiang, Meng Xiangming
Department of Chemistry, Anhui University, Hefei, 230601, China.
Dalton Trans. 2015 Apr 14;44(14):6613-9. doi: 10.1039/c5dt00012b.
A novel two-photon fluorescent probe () for hypochlorite (ClO(-)) has been developed based on specific ClO(-)-promoted oxidation of a C[double bond, length as m-dash]N bond. The probe shows linear fluorescence responses to ClO(-) with 23.5-fold enhancement. Two-photon fluorescence detection signals of to ClO(-) were observed with high selectivity and sensitivity. The good biocompatibility guaranteed the use of to detect intracellular ClO(-) under two-photon excitation. Moreover, has been successfully applied to monitor the endogenously produced hypochlorite in living cells (BV-2) stimulated by lipopolysaccharide (LPS). provides a new ClO(-) detection agent for the discovery of pharmacological microglia modulators by high-throughput screening.
基于次氯酸盐（ClO(-)）促进的C=N键特定氧化反应，开发了一种新型的用于检测次氯酸盐（ClO(-)）的双光子荧光探针（）。该探针对ClO(-)呈现线性荧光响应，增强了23.5倍。观察到对ClO(-)的双光子荧光检测信号具有高选择性和高灵敏度。良好的生物相容性保证了在双光子激发下用于检测细胞内ClO(-)。此外，已成功应用于监测脂多糖（LPS）刺激的活细胞（BV-2）中内源性产生的次氯酸盐。为通过高通量筛选发现药理学小胶质细胞调节剂提供了一种新的ClO(-)检测剂。
