A two-photon fluorescent probe for detecting endogenous hypochlorite in living cells.

A novel two-photon fluorescent probe () for hypochlorite (ClO(-)) has been developed based on specific ClO(-)-promoted oxidation of a C[double bond, length as m-dash]N bond. The probe shows linear fluorescence responses to ClO(-) with 23.5-fold enhancement. Two-photon fluorescence detection signals of to ClO(-) were observed with high selectivity and sensitivity. The good biocompatibility guaranteed the use of to detect intracellular ClO(-) under two-photon excitation. Moreover, has been successfully applied to monitor the endogenously produced hypochlorite in living cells (BV-2) stimulated by lipopolysaccharide (LPS). provides a new ClO(-) detection agent for the discovery of pharmacological microglia modulators by high-throughput screening.