Wang Kangnan, Sun Pengzhen, Chao Xijuan, Cao Duxia, Mao Zongwan, Liu Zhiqiang
School of Materials Science and Engineering, University of Jinan Jinan 250022 PR China
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University Guangzhou 510275 PR China
RSC Adv. 2018 Feb 13;8(13):6904-6909. doi: 10.1039/c8ra00093j. eCollection 2018 Feb 9.
Selective and sensitive fluorescent probes for ClO are desirable due to the importance of ClO in biological processes. Here, a coumarin Schiff's base, compound 1, has been developed and successfully used as a one- and two-photon fluorescent probe for ClO with high selectivity. This probe can recognize ClO with obvious color change from yellow-green to colorless and green to blue fluorescence emission, which can be observed by the naked eye. The properties of low cytotoxicity and good cell permeability allow it to be used for ClO detection in living cells and zebrafish by both one- and two-photon microscopy imaging. All these results indicate that the compound is a sensitive probe with potential for analysis of ClO in biological samples. The mechanism by which probe 1 recognizes ClO is possibly nucleophilic addition followed by hydrolysis.
由于次氯酸根（ClO）在生物过程中的重要性，因此需要选择性和灵敏的ClO荧光探针。在此，一种香豆素席夫碱化合物1已被开发出来，并成功用作对ClO具有高选择性的单光子和双光子荧光探针。该探针可以识别ClO，伴随着明显的颜色变化，从黄绿色变为无色，荧光发射从绿色变为蓝色，肉眼即可观察到。低细胞毒性和良好的细胞渗透性使其能够通过单光子和双光子显微镜成像用于活细胞和斑马鱼中ClO的检测。所有这些结果表明，该化合物是一种灵敏的探针，具有分析生物样品中ClO的潜力。探针1识别ClO的机制可能是亲核加成后水解。
