MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
Biomaterials. 2015;53:285-95. doi: 10.1016/j.biomaterials.2015.02.106. Epub 2015 Mar 17.
Endogenous hypochlorite ion (ClO(-)) is a highly reactive oxygen species (ROS) that is produced from hydrogen peroxide and chloride ions catalyzed by myeloperoxidase (MPO). And mitochondrion is one of the major sources of ROS including ClO(-). In the present work, a two-photon phosphorescent probe for ClO(-) in mitochondria was developed. An iridium(III) complex bearing a diaminomaleonitrile group as ClO(-) reactive moiety specifically responded to ClO(-) over other ions and ROSs. When the probe was reacted with ClO(-) to form an oxidized carboxylate product, a significant enhancement in phosphorescence intensity was observed under one-photon (402 nm) and two-photon (750 nm) excitation, with a two-photon absorption cross-section of 78.1 GM at 750 nm. More importantly, ICP-MS results and cellular images co-stained with Mito-tracker Green demonstrated that this probe possessed high specificity for mitochondria. This probe was applied in the one- and two-photon imaging of ClO(-) in vitro and in vivo. The results suggested endotoxin lipopolysaccharide (LPS) induced ClO(-) mostly generated in the liver of zebrafish.
内源性次氯酸根离子 (ClO(-)) 是一种高反应性的氧自由基 (ROS)，由过氧化物酶 (MPO) 催化的过氧化氢和氯离子产生。线粒体是 ROS 包括 ClO(-) 的主要来源之一。在本工作中，开发了一种用于线粒体中 ClO(-) 的双光子磷光探针。一个含有二氨基马来腈基团的铱 (III) 配合物作为 ClO(-) 反应部分，特异性地响应 ClO(-)，而不是其他离子和 ROS。当探针与 ClO(-)反应形成氧化的羧酸盐产物时，在单光子 (402nm) 和双光子 (750nm) 激发下观察到磷光强度显著增强，在 750nm 处的双光子吸收截面为 78.1GM。更重要的是，ICP-MS 结果和与 Mito-tracker Green 共染色的细胞图像表明，该探针对线粒体具有高特异性。该探针用于体外和体内 ClO(-)的单光子和双光子成像。结果表明，内毒素脂多糖 (LPS) 诱导的 ClO(-)主要在斑马鱼的肝脏中产生。
