Wiedenmann B, Huttner W B
Department of Internal Medicine, University of Heidelberg Medical School, Federal Republic of Germany.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;58(2):95-121. doi: 10.1007/BF02890062.
Normal and neoplastic neuroendocrine (NE) cells have been identified for many years by morphological criteria only. With the advent of immunocytochemistry, antibodies against NE-specific polypeptides have been used to identify NE cells that had been missed by conventional techniques, thus improving the diagnosis of NE cells. In this review article we discuss (i) the biochemical, cell biological and molecular biological data obtained so far for two major types of NE markers, synaptophysin, which is characteristic of the small "transparent-looking" neurosecretory vesicles, and the chromogranins/secretogranins, which are widespread constituents of the larger "dense-cored" secretory granules; (ii) the immunohistochemical data obtained for these marker proteins in normal and neoplastic human NE cells and tissues; and (iii) future possible developments involving these as well as other proteins that are associated with these two distinct secretory organelles of NE cells and may serve as potential markers in NE cell diagnosis.
多年来，正常和肿瘤性神经内分泌（NE）细胞一直仅通过形态学标准来识别。随着免疫细胞化学的出现，针对NE特异性多肽的抗体已被用于识别传统技术遗漏的NE细胞，从而改善了NE细胞的诊断。在这篇综述文章中，我们讨论：（i）到目前为止，针对两种主要类型的NE标志物获得的生化、细胞生物学和分子生物学数据，即小的“看起来透明”的神经分泌囊泡所特有的突触素，以及较大的“致密核心”分泌颗粒中广泛存在的嗜铬粒蛋白/分泌粒蛋白；（ii）在正常和肿瘤性人类NE细胞及组织中针对这些标志物蛋白获得的免疫组织化学数据；以及（iii）涉及这些以及与NE细胞这两种不同分泌细胞器相关的其他蛋白质的未来可能发展，这些蛋白质可能作为NE细胞诊断中的潜在标志物。
